Bioinformatics analyses and in vitro evidence for five and six stacked G-quadruplex forming sequences

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F18%3AA1901UJS" target="_blank" >RIV/61988987:17310/18:A1901UJS - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/18:00491059 RIV/86652079:_____/18:00491059

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.biochi.2018.05.002" target="_blank" >http://dx.doi.org/10.1016/j.biochi.2018.05.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biochi.2018.05.002" target="_blank" >10.1016/j.biochi.2018.05.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bioinformatics analyses and in vitro evidence for five and six stacked G-quadruplex forming sequences

Popis výsledku v původním jazyce

Quadruplexes are noncanonical DNA structures that arise in guanine rich loci and have important bio-logical functions. Classically, quadruplexes contain four stacked intramolecular G-tetrads. Surprisingly,although some algorithms allow searching for longer than 4G tracts for quadruplex formation, these havenot yet been systematically studied. Therefore, we analyzed the human genome for sequences that arepredicted to adopt stacked intramolecular G-tetrads with greater than four stacks. The data provideevidence for numerous G-quadruplexes that contain five or six stacked intramolecular G-tetrads. Thesesequences are predominantly found in known gene regulatory regions. Electrophoretic mobility assaysand circular dichroism spectroscopy indicate that these sequences form quadruplex structures in vitrounder physiological conditions. The localization and in vitro stability of these G-quadruplexes indicatetheir potentially important roles in gene regulation and their potential for therapeutic applications.

Název v anglickém jazyce

Bioinformatics analyses and in vitro evidence for five and six stacked G-quadruplex forming sequences

Popis výsledku anglicky

Quadruplexes are noncanonical DNA structures that arise in guanine rich loci and have important bio-logical functions. Classically, quadruplexes contain four stacked intramolecular G-tetrads. Surprisingly,although some algorithms allow searching for longer than 4G tracts for quadruplex formation, these havenot yet been systematically studied. Therefore, we analyzed the human genome for sequences that arepredicted to adopt stacked intramolecular G-tetrads with greater than four stacks. The data provideevidence for numerous G-quadruplexes that contain five or six stacked intramolecular G-tetrads. Thesesequences are predominantly found in known gene regulatory regions. Electrophoretic mobility assaysand circular dichroism spectroscopy indicate that these sequences form quadruplex structures in vitrounder physiological conditions. The localization and in vitro stability of these G-quadruplexes indicatetheir potentially important roles in gene regulation and their potential for therapeutic applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BIOCHIMIE

ISSN

0300-9084

e-ISSN

—

Svazek periodika

150

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

6

Strana od-do

70-75

Kód UT WoS článku

000434053900009

EID výsledku v databázi Scopus

—