Biodegradation of Reactive Orange 16 azo dye by simultaneous action of Pleurotus ostreatus and the yeast Candida zeylanoides

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F20%3AA21024V6" target="_blank" >RIV/61988987:17310/20:A21024V6 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/20:00533357 RIV/61989100:27640/20:10243416

Výsledek na webu

<a href="https://doi.org/10.1007/s12223-019-00767-3" target="_blank" >https://doi.org/10.1007/s12223-019-00767-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-019-00767-3" target="_blank" >10.1007/s12223-019-00767-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biodegradation of Reactive Orange 16 azo dye by simultaneous action of Pleurotus ostreatus and the yeast Candida zeylanoides

Popis výsledku v původním jazyce

The purpose was to investigate a simultaneous biodegradation of the recalcitrant monoazo dye Reactive Orange 16 (RO16) in a mixed culture consisting of a biofilm of Pleurotus ostreatus-colonizing polyamide carrier and a suspension of the yeast Candida zeylanoides to see their biological interactions and possible synergistic action during degradation. Decolorization in the mixed culture was more effective than in the fungal monoculture, the respective decolorizations reaching 87.5% and 70% on day 11. The proliferation of yeast was reduced compared with the C. zeylanoides monoculture but enabled the yeast to participate in decolorization. The interaction of P. ostreatus with the yeast resulted in a gradual decrease of fungal manganese-dependent peroxidase (MnP) and laccase activities. Gas chromatography-mass spectrometry (GC-MS) analysis of the degradation products brought evidence that P. ostreatus split the dye molecule asymmetrically to provide 4-(ethenylsulfonyl) benzene whose concentration was much decreased in the mixed culture suggesting its increased metabolization in the presence of the yeast. In contrast, C. zeylanoides split the azo bond symmetrically producing the metabolites 4-(ethenylsulfonyl) aniline and alpha-hydroxybenzenepropanoic acid. Those metabolites were rapidly degraded in the mixed culture. A novel aspect is represented by the evidence of a mutual cooperative action of the fungal and yeast microorganisms in the mixed culture resulting in rapid decolorization and degradation of the dye.

Název v anglickém jazyce

Biodegradation of Reactive Orange 16 azo dye by simultaneous action of Pleurotus ostreatus and the yeast Candida zeylanoides

Popis výsledku anglicky

The purpose was to investigate a simultaneous biodegradation of the recalcitrant monoazo dye Reactive Orange 16 (RO16) in a mixed culture consisting of a biofilm of Pleurotus ostreatus-colonizing polyamide carrier and a suspension of the yeast Candida zeylanoides to see their biological interactions and possible synergistic action during degradation. Decolorization in the mixed culture was more effective than in the fungal monoculture, the respective decolorizations reaching 87.5% and 70% on day 11. The proliferation of yeast was reduced compared with the C. zeylanoides monoculture but enabled the yeast to participate in decolorization. The interaction of P. ostreatus with the yeast resulted in a gradual decrease of fungal manganese-dependent peroxidase (MnP) and laccase activities. Gas chromatography-mass spectrometry (GC-MS) analysis of the degradation products brought evidence that P. ostreatus split the dye molecule asymmetrically to provide 4-(ethenylsulfonyl) benzene whose concentration was much decreased in the mixed culture suggesting its increased metabolization in the presence of the yeast. In contrast, C. zeylanoides split the azo bond symmetrically producing the metabolites 4-(ethenylsulfonyl) aniline and alpha-hydroxybenzenepropanoic acid. Those metabolites were rapidly degraded in the mixed culture. A novel aspect is represented by the evidence of a mutual cooperative action of the fungal and yeast microorganisms in the mixed culture resulting in rapid decolorization and degradation of the dye.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FOLIA MICROBIOLOGICA

ISSN

0015-5632

e-ISSN

1874-9356

Svazek periodika

65

Číslo periodika v rámci svazku

22.1.2020

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

629-638

Kód UT WoS článku

000508703100001

EID výsledku v databázi Scopus

2-s2.0-85078166363