Caterpillar gut and host plant phylloplane mycobiomes differ: a new perspective on fungal involvement in insect guts
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F20%3AA2102605" target="_blank" >RIV/61988987:17310/20:A2102605 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61388971:_____/20:00535349 RIV/00216208:11310/20:10422980
Výsledek na webu
<a href="https://academic.oup.com/femsec/article-abstract/96/9/fiaa116/5855491" target="_blank" >https://academic.oup.com/femsec/article-abstract/96/9/fiaa116/5855491</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/femsec/fiaa116" target="_blank" >10.1093/femsec/fiaa116</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Caterpillar gut and host plant phylloplane mycobiomes differ: a new perspective on fungal involvement in insect guts
Popis výsledku v původním jazyce
Compared with the highly diverse microbiota of leaves, herbivorous insects exhibit impoverished gut microbial communities. Research to date has focused on the bacterial component of these gut microbiomes, neglecting the fungal component. As caterpillar gut bacterial microbiomes are derived strongly from their diet, we hypothesized that their mycobiomes would reflect the host leaf mycobiomes. Using the ITS2 rDNA and V5–V6 16S rRNA gene regions for DNA metabarcoding of caterpillar gut and host leaf sample pairs we compared their mycobiome genus diversity and compositions and identified genera associated with caterpillar guts. Leaves and caterpillar guts harbored different mycobiomes with quite low qualitative similarity (Jaccard index = 38.03%). The fungal genera most significantly associated with the caterpillar gut included Penicillium, Mucor and unidentified Saccharomycetales, whereas leaf-associated genera included Holtermanniella, Gibberella (teleomorph of Fusarium) and Seimatosporium. Although caterpillar gut and leaf mycobiomes had similar genus richness overall, this indicator was not correlated for individual duplets. Moreover, as more samples entered the analysis, mycobiome richness increased more rapidly in caterpillar guts than in leaves. The results suggest that the mycobiota of the caterpillar gut differs from that of their feeding substrate; further, the mycobiomes appear to be richer than the well-studied bacterial microbiotas.
Název v anglickém jazyce
Caterpillar gut and host plant phylloplane mycobiomes differ: a new perspective on fungal involvement in insect guts
Popis výsledku anglicky
Compared with the highly diverse microbiota of leaves, herbivorous insects exhibit impoverished gut microbial communities. Research to date has focused on the bacterial component of these gut microbiomes, neglecting the fungal component. As caterpillar gut bacterial microbiomes are derived strongly from their diet, we hypothesized that their mycobiomes would reflect the host leaf mycobiomes. Using the ITS2 rDNA and V5–V6 16S rRNA gene regions for DNA metabarcoding of caterpillar gut and host leaf sample pairs we compared their mycobiome genus diversity and compositions and identified genera associated with caterpillar guts. Leaves and caterpillar guts harbored different mycobiomes with quite low qualitative similarity (Jaccard index = 38.03%). The fungal genera most significantly associated with the caterpillar gut included Penicillium, Mucor and unidentified Saccharomycetales, whereas leaf-associated genera included Holtermanniella, Gibberella (teleomorph of Fusarium) and Seimatosporium. Although caterpillar gut and leaf mycobiomes had similar genus richness overall, this indicator was not correlated for individual duplets. Moreover, as more samples entered the analysis, mycobiome richness increased more rapidly in caterpillar guts than in leaves. The results suggest that the mycobiota of the caterpillar gut differs from that of their feeding substrate; further, the mycobiomes appear to be richer than the well-studied bacterial microbiotas.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10618 - Ecology
Návaznosti výsledku
Projekt
<a href="/cs/project/GA18-08803S" target="_blank" >GA18-08803S: Mikrobiota symbiontů herbivorního hmyzu: klíč k pochopení hostitelské specializace?</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
FEMS Microbiology ecology
ISSN
0168-6496
e-ISSN
1574-6941
Svazek periodika
96
Číslo periodika v rámci svazku
9
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
11
Strana od-do
1-11
Kód UT WoS článku
000575458400003
EID výsledku v databázi Scopus
2-s2.0-85089617222