Trypanosoma cruzi strain and starvation-driven mitochondrial RNA editing and transcriptome variability

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F22%3AA2302GR4" target="_blank" >RIV/61988987:17310/22:A2302GR4 - isvavai.cz</a>

Výsledek na webu

<a href="https://rnajournal.cshlp.org/content/28/7/993" target="_blank" >https://rnajournal.cshlp.org/content/28/7/993</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1261/rna.079088.121" target="_blank" >10.1261/rna.079088.121</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Trypanosoma cruzi strain and starvation-driven mitochondrial RNA editing and transcriptome variability

Popis výsledku v původním jazyce

Trypanosoma cruzi is a unicellular protistan parasitic species that is comprised of strains and isolates exhibiting high levels of genetic and metabolic variability. In the insect vector, it is known to be highly responsive to starvation, a signal for progression to a life stage in which it can infect mammalian cells. Most mRNAs encoded in its mitochondrion require the targeted insertion and deletion of uridines to become translatable transcripts. This study defined differences in uridine-insertion/deletion RNA editing among three strains and established the mechanism whereby abundances of edited (and, thus, translatable) mitochondrial gene products increase during starvation. Our approach utilized our custom T-Aligner toolkit to describe transcriptome-wide editing events and reconstruct editing products from high-throughput sequencing data.

Název v anglickém jazyce

Trypanosoma cruzi strain and starvation-driven mitochondrial RNA editing and transcriptome variability

Popis výsledku anglicky

Trypanosoma cruzi is a unicellular protistan parasitic species that is comprised of strains and isolates exhibiting high levels of genetic and metabolic variability. In the insect vector, it is known to be highly responsive to starvation, a signal for progression to a life stage in which it can infect mammalian cells. Most mRNAs encoded in its mitochondrion require the targeted insertion and deletion of uridines to become translatable transcripts. This study defined differences in uridine-insertion/deletion RNA editing among three strains and established the mechanism whereby abundances of edited (and, thus, translatable) mitochondrial gene products increase during starvation. Our approach utilized our custom T-Aligner toolkit to describe transcriptome-wide editing events and reconstruct editing products from high-throughput sequencing data.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RNA

ISSN

1355-8382

e-ISSN

1469-9001

Svazek periodika

—

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

19

Strana od-do

993-1012

Kód UT WoS článku

000812825700006

EID výsledku v databázi Scopus

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