Lipid Phase Behaviour of the Curvature Region of Thylakoid Membranes of <scp><i>Spinacia oleracea</i></scp>

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F25%3AA2603B4B" target="_blank" >RIV/61988987:17310/25:A2603B4B - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1111/ppl.70289" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/ppl.70289</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/ppl.70289" target="_blank" >10.1111/ppl.70289</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lipid Phase Behaviour of the Curvature Region of Thylakoid Membranes of <scp><i>Spinacia oleracea</i></scp>

Popis výsledku v původním jazyce

Thylakoid membranes (TMs) of oxygenic photosynthetic organisms are flat membrane vesicles, which form highly organised, interconnected membrane networks. In vascular plants, they are differentiated into stacked and unstacked regions, the grana and stroma lamellae, respectively; they are densely packed with protein complexes performing the light reactions of photosynthesis and generating a proton motive force (pmf). The maintenance of pmf and its utilisation for ATP synthesis requires sealing the TMs at their highly curved regions (CRs). These regions are devoid of chlorophyll‐containing proteins but contain the curvature‐inducing CURVATURE THYLAKOID1 (CURT1) proteins and are enriched in lipids. Because of the highly curved nature of this region, at the margins of grana and stroma TMs, the molecular organisation of lipid molecules is likely to possess distinct features compared to those in the major TM domains. To clarify this question, we isolated CR fractions from Spinacia oleracea</jats:italic></jats:styled-content> and, using BN‐PAGE and western blot analysis, verified that they are enriched in CURT1 proteins and in lipids. The lipid phase behaviour of these fractions was fingerprinted with P‐NMR spectroscopy, which revealed that the bulk lipid molecules assume a non‐bilayer, isotropic lipid phase. This finding underpins the importance of the main, non‐bilayer lipid species, monogalactosyldiacylglycerol, of TMs in their self‐assembly and functional activity.

Název v anglickém jazyce

Lipid Phase Behaviour of the Curvature Region of Thylakoid Membranes of <scp><i>Spinacia oleracea</i></scp>

Popis výsledku anglicky

Thylakoid membranes (TMs) of oxygenic photosynthetic organisms are flat membrane vesicles, which form highly organised, interconnected membrane networks. In vascular plants, they are differentiated into stacked and unstacked regions, the grana and stroma lamellae, respectively; they are densely packed with protein complexes performing the light reactions of photosynthesis and generating a proton motive force (pmf). The maintenance of pmf and its utilisation for ATP synthesis requires sealing the TMs at their highly curved regions (CRs). These regions are devoid of chlorophyll‐containing proteins but contain the curvature‐inducing CURVATURE THYLAKOID1 (CURT1) proteins and are enriched in lipids. Because of the highly curved nature of this region, at the margins of grana and stroma TMs, the molecular organisation of lipid molecules is likely to possess distinct features compared to those in the major TM domains. To clarify this question, we isolated CR fractions from Spinacia oleracea</jats:italic></jats:styled-content> and, using BN‐PAGE and western blot analysis, verified that they are enriched in CURT1 proteins and in lipids. The lipid phase behaviour of these fractions was fingerprinted with P‐NMR spectroscopy, which revealed that the bulk lipid molecules assume a non‐bilayer, isotropic lipid phase. This finding underpins the importance of the main, non‐bilayer lipid species, monogalactosyldiacylglycerol, of TMs in their self‐assembly and functional activity.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/GA23-07744S" target="_blank" >GA23-07744S: Identifikace a charakterizace struktur souvisejících s odlišnými fázemi lipidů tylakoidních membrán rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiologia Plantarum

ISSN

0031-9317

e-ISSN

1399-3054

Svazek periodika

—

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

—

Kód UT WoS článku

001509967400001

EID výsledku v databázi Scopus

2-s2.0-105008235513