Revisiting the QA model of chlorophyll-a fluorescence induction: new perspectives to monitor the photochemical activity and structural dynamics of photosystem II

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F25%3AA2603D1C" target="_blank" >RIV/61988987:17310/25:A2603D1C - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/10.1007/s11120-025-01178-x" target="_blank" >https://link.springer.com/10.1007/s11120-025-01178-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11120-025-01178-x" target="_blank" >10.1007/s11120-025-01178-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Revisiting the QA model of chlorophyll-a fluorescence induction: new perspectives to monitor the photochemical activity and structural dynamics of photosystem II

Popis výsledku v původním jazyce

The technique of chlorophyll-a fluorescence induction (ChlF) is widely used in plant biology. The 'mainstream', so-called Q(A) model of ChlF, posits that the reaction centers (RCs) of Photosystem-II (PSII) exist in two states, quenched (F-o) or open (PSIIO), and unquenched (F-m) or closed (PSIIC), containing the primary quinone acceptor, Q(A), in oxidized and reduced state, respectively; and that the quantum yield of PSII photochemistry of a dark-adapted sample is Y(II) = F-v/F-m, where F-v=F-m-F-o. The widespread application of ChlF, with user-friendly instruments, and the use of the Q(A) model, have substantially contributed to our understanding of the operation of the photosynthetic machineries under different environmental conditions. However, recent experimental data - multiple light-induced fluorescence increments in PSIIC; the complex, pH and temperature dependent kinetic and spectral features of key ChlF parameters; twith enhanced stabilization of the charges - cannot be reconciled with the Q(A) model. These features are explained by subtle conformational transitions driven by stationary and transient electric fields and associated dielectric relaxation processes. This interpretation, while invites further studies, places the hitherto unknown structural and functional plasticity of the RC matrix in the context of its physiological significance.

Název v anglickém jazyce

Revisiting the QA model of chlorophyll-a fluorescence induction: new perspectives to monitor the photochemical activity and structural dynamics of photosystem II

Popis výsledku anglicky

The technique of chlorophyll-a fluorescence induction (ChlF) is widely used in plant biology. The 'mainstream', so-called Q(A) model of ChlF, posits that the reaction centers (RCs) of Photosystem-II (PSII) exist in two states, quenched (F-o) or open (PSIIO), and unquenched (F-m) or closed (PSIIC), containing the primary quinone acceptor, Q(A), in oxidized and reduced state, respectively; and that the quantum yield of PSII photochemistry of a dark-adapted sample is Y(II) = F-v/F-m, where F-v=F-m-F-o. The widespread application of ChlF, with user-friendly instruments, and the use of the Q(A) model, have substantially contributed to our understanding of the operation of the photosynthetic machineries under different environmental conditions. However, recent experimental data - multiple light-induced fluorescence increments in PSIIC; the complex, pH and temperature dependent kinetic and spectral features of key ChlF parameters; twith enhanced stabilization of the charges - cannot be reconciled with the Q(A) model. These features are explained by subtle conformational transitions driven by stationary and transient electric fields and associated dielectric relaxation processes. This interpretation, while invites further studies, places the hitherto unknown structural and functional plasticity of the RC matrix in the context of its physiological significance.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/GA23-07744S" target="_blank" >GA23-07744S: Identifikace a charakterizace struktur souvisejících s odlišnými fázemi lipidů tylakoidních membrán rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Photosynthesis Research

ISSN

0166-8595

e-ISSN

1573-5079

Svazek periodika

—

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

14

Strana od-do

—

Kód UT WoS článku

001600283900001

EID výsledku v databázi Scopus

2-s2.0-105019487698