Permeability measurements of biological membranes using the surface plasmon resonance imaging method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27360%2F21%3A10248476" target="_blank" >RIV/61989100:27360/21:10248476 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989100:27710/21:10248476

Výsledek na webu

<a href="https://secure.confis.cz/chisa2021-virtually" target="_blank" >https://secure.confis.cz/chisa2021-virtually</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Permeability measurements of biological membranes using the surface plasmon resonance imaging method

Popis výsledku v původním jazyce

Abstract in conference proceedings. CHISA 2021, 15.-18. 3. 2021, virtually. Membrane processes are innovative and efficient separation methods, using different molecular properties of separated substances. An essential element of every membrane process is the membrane, a selective barrier between the two environments that allow the transport of selected particles. A property typical of membranes is their permeability. The measurement of the permeability of semipermeable membranes is currently performed by several known methods, the most well-known of which is the use of Franz diffusion cells. The basic idea is to use the diffusion of the permeant in two cells, separated from each other by a membrane of known area, wherein one cell there is a pure solvent and in the other a solvent with the appropriate diffusing substance. Permeability is evaluated based on the time dependence of changes in concentrations in both cells. The surface plasmon resonance imaging (SPRi) method is a rapidly evolving optical analytical technique in which we use online detection of changes in the concentration of selected permeants over time, and thus allows obtaining parameters for rapid and effective evaluation of membrane permeability for selected substances. Specifically, a change in the signal depending on the change in the concentration of the measured analyte (permeant) in the flow cell is detected. The time response of the signal obtained at the detection spots on the surface of the measuring chip plots a curve in the sensorgram, the first derivative of which is used as a parameter for the calculation of individual permeabilities. For measurement purposes, known xenobiotics occurring in surface and groundwater, such as paracetamol, urea, or diuron, are used as permeants.

Název v anglickém jazyce

Permeability measurements of biological membranes using the surface plasmon resonance imaging method

Popis výsledku anglicky

Abstract in conference proceedings. CHISA 2021, 15.-18. 3. 2021, virtually. Membrane processes are innovative and efficient separation methods, using different molecular properties of separated substances. An essential element of every membrane process is the membrane, a selective barrier between the two environments that allow the transport of selected particles. A property typical of membranes is their permeability. The measurement of the permeability of semipermeable membranes is currently performed by several known methods, the most well-known of which is the use of Franz diffusion cells. The basic idea is to use the diffusion of the permeant in two cells, separated from each other by a membrane of known area, wherein one cell there is a pure solvent and in the other a solvent with the appropriate diffusing substance. Permeability is evaluated based on the time dependence of changes in concentrations in both cells. The surface plasmon resonance imaging (SPRi) method is a rapidly evolving optical analytical technique in which we use online detection of changes in the concentration of selected permeants over time, and thus allows obtaining parameters for rapid and effective evaluation of membrane permeability for selected substances. Specifically, a change in the signal depending on the change in the concentration of the measured analyte (permeant) in the flow cell is detected. The time response of the signal obtained at the detection spots on the surface of the measuring chip plots a curve in the sensorgram, the first derivative of which is used as a parameter for the calculation of individual permeabilities. For measurement purposes, known xenobiotics occurring in surface and groundwater, such as paracetamol, urea, or diuron, are used as permeants.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20700 - Environmental engineering

Projekt

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Návaznosti

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Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů