Methods for analysis of human amniotic fluids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27640%2F14%3A86091453" target="_blank" >RIV/61989100:27640/14:86091453 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Methods for analysis of human amniotic fluids

Popis výsledku v původním jazyce

Analysis of bodyfluids is new interesting approach for researching air-pollution impact on human health. Bodyfluids make an internal environment of tissues and organs and therefore bodyfluids composition can affect their functions. Bodyfluids are in touch with outer environment mostly through the respiratory tract, and thus inhalation of polluted air may lead to penetration of some chemicals into the bodyfluids. Air-polluting suspended solid particles are able to penetrate biomembranes due to their size below 100nm. One of these biomembranes is placenta, which prevents from penetrating harmful substances to amniotic fluid. Amniotic fluid provides fetus protection and fetus nutrition from mother's blood. Therefore, its composition is crucial for fetus development. Collected samples of human amniotic fluid were analyzed using the selected techniques: Raman microspectroscopy, Scanning electron microscopy with energy-dispersed spectrometer (SEM-EDS), Fourier transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis. Major natural compounds in human amniotic fluid are sodium chloride and organic compounds, mainly proteins, that were analyzed by XRD, SEM-EDS, FTIR and Raman microspectroscopy. In collected samples were also found metal-based particles. These results were obtained by SEM-EDS and Raman microspectroscopy. SEM-EDS analysis seems to be most suitable, because it is quite fast and precise data obtaining. Compared to Raman microspectroscopy, which is more time-consuming method. However, Raman microspectroscopy is a valuable tool for point-phase-analysis. XRD wasn't able to detect metal and its compounds presence, because of its detection limit.

Název v anglickém jazyce

Methods for analysis of human amniotic fluids

Popis výsledku anglicky

Analysis of bodyfluids is new interesting approach for researching air-pollution impact on human health. Bodyfluids make an internal environment of tissues and organs and therefore bodyfluids composition can affect their functions. Bodyfluids are in touch with outer environment mostly through the respiratory tract, and thus inhalation of polluted air may lead to penetration of some chemicals into the bodyfluids. Air-polluting suspended solid particles are able to penetrate biomembranes due to their size below 100nm. One of these biomembranes is placenta, which prevents from penetrating harmful substances to amniotic fluid. Amniotic fluid provides fetus protection and fetus nutrition from mother's blood. Therefore, its composition is crucial for fetus development. Collected samples of human amniotic fluid were analyzed using the selected techniques: Raman microspectroscopy, Scanning electron microscopy with energy-dispersed spectrometer (SEM-EDS), Fourier transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis. Major natural compounds in human amniotic fluid are sodium chloride and organic compounds, mainly proteins, that were analyzed by XRD, SEM-EDS, FTIR and Raman microspectroscopy. In collected samples were also found metal-based particles. These results were obtained by SEM-EDS and Raman microspectroscopy. SEM-EDS analysis seems to be most suitable, because it is quite fast and precise data obtaining. Compared to Raman microspectroscopy, which is more time-consuming method. However, Raman microspectroscopy is a valuable tool for point-phase-analysis. XRD wasn't able to detect metal and its compounds presence, because of its detection limit.

Druh

D - Stať ve sborníku

CEP obor

DN - Vliv životního prostředí na zdraví

OECD FORD obor

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Projekt

<a href="/cs/project/EE.2.3.20.0074" target="_blank" >EE.2.3.20.0074: Nanotechnologie - báze pro mezinárodní spolupráci</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2013 : 5th international conference : October 16th-18th 2013, hotel Voroněž I, Brno, Czech Republic, EU : conference proceedings [CD-ROM]

ISBN

978-80-87294-47-5

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

681-686

Název nakladatele

Tanger

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

16. 10. 2013

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000352070900121