Raman microspectroscopy as a useful tool for nanopathology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F17%3AA1801RC5" target="_blank" >RIV/61988987:17110/17:A1801RC5 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989100:27640/17:86098436 RIV/61989100:27740/17:86098436 RIV/00843989:_____/17:E0106163

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jrs.5045" target="_blank" >http://dx.doi.org/10.1002/jrs.5045</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jrs.5045" target="_blank" >10.1002/jrs.5045</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Raman microspectroscopy as a useful tool for nanopathology

Popis výsledku v původním jazyce

Interactions between organisms and micro-and nano-sized particles are studied in a relatively new research fieldnanopathology. Some diseases (oncological and/or inflammatory) may be connected with accumulation of the particles in the tissues. In general, precise and accurate qualitative evaluation of chemical composition of particles is very important. In such analyses, scanning electron microscopy with energy dispersive X-ray spectroscopy is used most often. Nevertheless, it reveals merely the elemental composition of particles/clusters. On the other hand, because Raman microspectroscopy is nondestructive and it is coupled with an optical microscope, it has potential for detection of particles of unknown nature; in addition, it allows determination of the chemical composition of these particles/clusters. Therefore, this technique could be a very useful supporting tool for nanopathology. The aim of the presented study is to survey the possibilities and advantages of Raman microspectroscopy for the detection and characterization of particles of an unknown nature in the samples of human nasal mucosa and amniotic fluids. Three different approaches of analysis are hereby discussed: (1) analysis of the entire area of the sample, (2) division of the sample in sections, and subsequent analysis of a defined number of points in selected parts, and (3) Raman spectral imaging of the selected parts. Results clearly prove high efficiency of Raman microspectroscopy as a tool for characterization of the particles/clusters in human tissues and body fluids, and thus it may allow a better understanding of the potential impact to human health in relation to specific diagnoses. Copyright (c) 2016 John Wiley & Sons, Ltd.

Název v anglickém jazyce

Raman microspectroscopy as a useful tool for nanopathology

Popis výsledku anglicky

Interactions between organisms and micro-and nano-sized particles are studied in a relatively new research fieldnanopathology. Some diseases (oncological and/or inflammatory) may be connected with accumulation of the particles in the tissues. In general, precise and accurate qualitative evaluation of chemical composition of particles is very important. In such analyses, scanning electron microscopy with energy dispersive X-ray spectroscopy is used most often. Nevertheless, it reveals merely the elemental composition of particles/clusters. On the other hand, because Raman microspectroscopy is nondestructive and it is coupled with an optical microscope, it has potential for detection of particles of unknown nature; in addition, it allows determination of the chemical composition of these particles/clusters. Therefore, this technique could be a very useful supporting tool for nanopathology. The aim of the presented study is to survey the possibilities and advantages of Raman microspectroscopy for the detection and characterization of particles of an unknown nature in the samples of human nasal mucosa and amniotic fluids. Three different approaches of analysis are hereby discussed: (1) analysis of the entire area of the sample, (2) division of the sample in sections, and subsequent analysis of a defined number of points in selected parts, and (3) Raman spectral imaging of the selected parts. Results clearly prove high efficiency of Raman microspectroscopy as a tool for characterization of the particles/clusters in human tissues and body fluids, and thus it may allow a better understanding of the potential impact to human health in relation to specific diagnoses. Copyright (c) 2016 John Wiley & Sons, Ltd.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30109 - Pathology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF RAMAN SPECTROSCOPY

ISSN

0377-0486

e-ISSN

1097-4555

Svazek periodika

48

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

357-362

Kód UT WoS článku

000396657800001

EID výsledku v databázi Scopus

2-s2.0-84991325407