DEVELOPMENT LC-MS/MS METHOD FOR DETERMINATION ANTIEPILEPTICS IN REAL WATER SAMPLES

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27710%2F22%3A10251514" target="_blank" >RIV/61989100:27710/22:10251514 - isvavai.cz</a>

Výsledek na webu

<a href="https://dspace.vsb.cz/handle/10084/149048" target="_blank" >https://dspace.vsb.cz/handle/10084/149048</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

DEVELOPMENT LC-MS/MS METHOD FOR DETERMINATION ANTIEPILEPTICS IN REAL WATER SAMPLES

Popis výsledku v původním jazyce

Emerging contaminants include pharmaceuticals, hormones, and personal care products that appear insurface, ground, and drinking water. Protection of water resources is a worldwide concern due to the use of waterfor human activities. Indeed, water pollution severely limits access to drinking water for human populations andrepresents a major cause of death. During their lifetime, humans consume many pharmaceuticals that are excretedform the body in the urine and subsequently enter groundwaters and rivers. This work describes the development,implementation and validation of highly selectivity and sensitive analytical method by liquid chromatography withtandem mass spectrometry (LC-MS/MS) that uses sample pre-treatment by solid phase extraction (SPE). PlexaPCX columns (Agilent) were used for SPE. Kinetex Phenyl-Hexyl chromatography column (Phenomenex) was usedfor analytical separation of the compound. Subsequent detection was performed on a Sciex QTrap 6500+ tandemmass spectrometry (MS/MS) using MRM mode. Quantitative analysis was performed using the internal standard(IS) methods. Subsequently, the implemented method was successfully validated for 19 antiepileptics drugs. Thelimit of detection (LOD) was in the range of 0.4-250 pg/ml. The successfully valeted method was successfullyapplied to the analysis of real water sample in the vicinity of the cities Ostrava and Bílovec, Czech Republic.

Název v anglickém jazyce

DEVELOPMENT LC-MS/MS METHOD FOR DETERMINATION ANTIEPILEPTICS IN REAL WATER SAMPLES

Popis výsledku anglicky

Emerging contaminants include pharmaceuticals, hormones, and personal care products that appear insurface, ground, and drinking water. Protection of water resources is a worldwide concern due to the use of waterfor human activities. Indeed, water pollution severely limits access to drinking water for human populations andrepresents a major cause of death. During their lifetime, humans consume many pharmaceuticals that are excretedform the body in the urine and subsequently enter groundwaters and rivers. This work describes the development,implementation and validation of highly selectivity and sensitive analytical method by liquid chromatography withtandem mass spectrometry (LC-MS/MS) that uses sample pre-treatment by solid phase extraction (SPE). PlexaPCX columns (Agilent) were used for SPE. Kinetex Phenyl-Hexyl chromatography column (Phenomenex) was usedfor analytical separation of the compound. Subsequent detection was performed on a Sciex QTrap 6500+ tandemmass spectrometry (MS/MS) using MRM mode. Quantitative analysis was performed using the internal standard(IS) methods. Subsequently, the implemented method was successfully validated for 19 antiepileptics drugs. Thelimit of detection (LOD) was in the range of 0.4-250 pg/ml. The successfully valeted method was successfullyapplied to the analysis of real water sample in the vicinity of the cities Ostrava and Bílovec, Czech Republic.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20400 - Chemical engineering

Projekt

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Návaznosti

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Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů