Nucleotides in Erythrocytes - Incorporation Study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F05%3A10213690" target="_blank" >RIV/61989592:15110/05:10213690 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Nucleotides in Erythrocytes - Incorporation Study

Popis výsledku v původním jazyce

Objective: Analysis of nucleotides for diagnosing inborn errors of metabolism. Transport studies of purine ribosides into erythrocytes. Material and Methods: Erythrocytes were incubated with purine ribosides. Ribosides were transported through erythrocyte membrane and converted into ribotides. Nucleotides were separated and identified by capillary electrophoretic method using background electrolyte 40 mmol/L citric acid ? 0.8 mmol/L cetyltrimethylammonium bromide-gama-aminobutyric acid, pH 4.4. Results:We found accumulated deoxynucleotides and nucleotides in erythrocyte extracts, which mimics metabolic situation of enzyme defects of purine metabolism. Under optimal electrophoretic conditions all compounds of interest were separated. Conclusion: The method can be successfully used to analyze intracellular nucleotides and diagnose inherited disorders of nucleotide metabolism.

Název v anglickém jazyce

Nucleotides in Erythrocytes - Incorporation Study

Popis výsledku anglicky

Objective: Analysis of nucleotides for diagnosing inborn errors of metabolism. Transport studies of purine ribosides into erythrocytes. Material and Methods: Erythrocytes were incubated with purine ribosides. Ribosides were transported through erythrocyte membrane and converted into ribotides. Nucleotides were separated and identified by capillary electrophoretic method using background electrolyte 40 mmol/L citric acid ? 0.8 mmol/L cetyltrimethylammonium bromide-gama-aminobutyric acid, pH 4.4. Results:We found accumulated deoxynucleotides and nucleotides in erythrocyte extracts, which mimics metabolic situation of enzyme defects of purine metabolism. Under optimal electrophoretic conditions all compounds of interest were separated. Conclusion: The method can be successfully used to analyze intracellular nucleotides and diagnose inherited disorders of nucleotide metabolism.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/NR7796" target="_blank" >NR7796: DEFEKTY PURINOVÉ DE NOVO SYNTÉZY - METABOLICKÁ STUDIE</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Klinická biochemie a metabolismus

ISSN

1210-7921

e-ISSN

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Svazek periodika

13

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

2

Strana od-do

182-183

Kód UT WoS článku

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EID výsledku v databázi Scopus

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