Cytoprotektivní účinek extraktu z borůvek proti oxidačnímu poškození potkaních hepatocytů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F07%3A00003546" target="_blank" >RIV/61989592:15110/07:00003546 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cytoprotective effect of a bilberry extract against oxidative damage of rat hepatocytes

Popis výsledku v původním jazyce

The effect of a bilberry extract (BE, 25% anthocyanins) against oxidative damage in primary cultures of rat hepatocytes, induced by tert-butyl hydroperoxide and allyl alcohol, was investigated. BE displayed cytoprotective effects at 100 and 500 ?g/ml inthe MTT viability test. It protected the cells against lactate dehydrogenase leakage and lipoperoxidation products formation. Maximum protection (58%) was noted using 500 ?g/ml of BE and intoxication by allyl alcohol. The observed cytoprotective effect is probably due to the antioxidant properties of its constituents, mainly anthocyanins. BE scavenged DPPH (IC50 3.99 ? 0.14 ?g/ml) and enzymatically generated superoxide radical with an activity equivalent to 108 ? 7.2 U of superoxide dismutase per mg ofextract. Our results support the use of bilberry and bilberry extracts in functional foods and food supplements designed for the prevention of chronic diseases associated with oxidative stress.

Název v anglickém jazyce

Cytoprotective effect of a bilberry extract against oxidative damage of rat hepatocytes

Popis výsledku anglicky

The effect of a bilberry extract (BE, 25% anthocyanins) against oxidative damage in primary cultures of rat hepatocytes, induced by tert-butyl hydroperoxide and allyl alcohol, was investigated. BE displayed cytoprotective effects at 100 and 500 ?g/ml inthe MTT viability test. It protected the cells against lactate dehydrogenase leakage and lipoperoxidation products formation. Maximum protection (58%) was noted using 500 ?g/ml of BE and intoxication by allyl alcohol. The observed cytoprotective effect is probably due to the antioxidant properties of its constituents, mainly anthocyanins. BE scavenged DPPH (IC50 3.99 ? 0.14 ?g/ml) and enzymatically generated superoxide radical with an activity equivalent to 108 ? 7.2 U of superoxide dismutase per mg ofextract. Our results support the use of bilberry and bilberry extracts in functional foods and food supplements designed for the prevention of chronic diseases associated with oxidative stress.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/FT-TA3%2F024" target="_blank" >FT-TA3/024: Rostlinná chemoprotektiva - Nové doplňky stravy pro zdraví a prevenci před chronickými nemocemi.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Chemistry

ISSN

0308-8146

e-ISSN

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Svazek periodika

101

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

912-917

Kód UT WoS článku

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EID výsledku v databázi Scopus

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