Protectivity of Blue Honeysuckle Extract against Oxidative Human Endothelial Cells and Rat Hepatocytes Damage.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F09%3A00009524" target="_blank" >RIV/61989592:15110/09:00009524 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Protectivity of Blue Honeysuckle Extract against Oxidative Human Endothelial Cells and Rat Hepatocytes Damage.

Popis výsledku v původním jazyce

The effect of Lonicera caerulea L. (Blue Honeysuckle) phenolic fraction (18.5 % anthocyanins) on cell viability and against oxidative damage in low density lipoproteins (oxLDL), in rat microsomes and in primary cultures of rat hepatocytes and human umbilical vein endothelial cells (HUVEC), was tested. The phenolic fraction was non-toxic to rat hepatocytes and HUVEC at tested concentrations (1 ? 1000 ?g/mL) and time intervals up to 24 h inclusive. Phenolic fraction inhibited rat liver microsomes peroxidation, induced by tert-butyl hydroperoxide (tBH), with IC50 values 160 ? 20 ?g/mL. The fraction at 0.5, 1.0 and 2.0 ?g/mL delayed LDL oxidation, induced by Cu2+, by 130 ? 20%, 200 ? 30% and 400 ? 10%, respectively. The treatment of HUVEC with oxidativelymodified LDL induced an increase in LDH leakage and TBARS formation, and resulted in lower formazan formation from MTT uptake, most pronounced for 200 ?g/mL (24h oxidation) after 2 h incubation. The protective effect of the phenolic

Název v anglickém jazyce

Protectivity of Blue Honeysuckle Extract against Oxidative Human Endothelial Cells and Rat Hepatocytes Damage.

Popis výsledku anglicky

The effect of Lonicera caerulea L. (Blue Honeysuckle) phenolic fraction (18.5 % anthocyanins) on cell viability and against oxidative damage in low density lipoproteins (oxLDL), in rat microsomes and in primary cultures of rat hepatocytes and human umbilical vein endothelial cells (HUVEC), was tested. The phenolic fraction was non-toxic to rat hepatocytes and HUVEC at tested concentrations (1 ? 1000 ?g/mL) and time intervals up to 24 h inclusive. Phenolic fraction inhibited rat liver microsomes peroxidation, induced by tert-butyl hydroperoxide (tBH), with IC50 values 160 ? 20 ?g/mL. The fraction at 0.5, 1.0 and 2.0 ?g/mL delayed LDL oxidation, induced by Cu2+, by 130 ? 20%, 200 ? 30% and 400 ? 10%, respectively. The treatment of HUVEC with oxidativelymodified LDL induced an increase in LDH leakage and TBARS formation, and resulted in lower formazan formation from MTT uptake, most pronounced for 200 ?g/mL (24h oxidation) after 2 h incubation. The protective effect of the phenolic

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/FT-TA3%2F024" target="_blank" >FT-TA3/024: Rostlinná chemoprotektiva - Nové doplňky stravy pro zdraví a prevenci před chronickými nemocemi.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Agricultural and Food Chemistry

ISSN

0021-8561

e-ISSN

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Svazek periodika

57

Číslo periodika v rámci svazku

15

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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