The reduction of doxorubicin at a mercury electrode and monitoring its interaction with DNA using constant current chronopotentiometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F09%3A00009743" target="_blank" >RIV/61989592:15110/09:00009743 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/09:00343810

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

The reduction of doxorubicin at a mercury electrode and monitoring its interaction with DNA using constant current chronopotentiometry

Popis výsledku v původním jazyce

In this report, voltammetry with linear scan and chronopotentiometric stripping (CPS) with constant current were used for the analysis of doxorubicin (DOX) at a hanging mercury drop electrode (HMDE). CPS was used for the study of DOX in situ electrochemical reduction in adsorbed state and for ex situ (adsorptive transfer) analysis of the drug. For the first time, CPS was used to study the reversible reduction of the DOX quinine moiety at ?0.45 V (vs Ag|AgCl|3 M KCl) as well as electrode processes givingrise to an irreversible signal around ?1.45 V at the HMDE in 0.2 M acetate or Britton?Robinson buffers at different pH values. The dependence of the latter signal on pH revealed involvement of protonation equilibria; however, neither CV nor CPS data confirmed the catalytic character of the electrode reaction previously suggested by other authors. The CPS method was also applied to monitor the DOX interaction with double- (ds) and single-stranded (ss) DNA. In the presence of dsDNA, more

Název v anglickém jazyce

The reduction of doxorubicin at a mercury electrode and monitoring its interaction with DNA using constant current chronopotentiometry

Popis výsledku anglicky

In this report, voltammetry with linear scan and chronopotentiometric stripping (CPS) with constant current were used for the analysis of doxorubicin (DOX) at a hanging mercury drop electrode (HMDE). CPS was used for the study of DOX in situ electrochemical reduction in adsorbed state and for ex situ (adsorptive transfer) analysis of the drug. For the first time, CPS was used to study the reversible reduction of the DOX quinine moiety at ?0.45 V (vs Ag|AgCl|3 M KCl) as well as electrode processes givingrise to an irreversible signal around ?1.45 V at the HMDE in 0.2 M acetate or Britton?Robinson buffers at different pH values. The dependence of the latter signal on pH revealed involvement of protonation equilibria; however, neither CV nor CPS data confirmed the catalytic character of the electrode reaction previously suggested by other authors. The CPS method was also applied to monitor the DOX interaction with double- (ds) and single-stranded (ss) DNA. In the presence of dsDNA, more

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Collection of Czechoslovak Chemical Communications

ISSN

0010-0765

e-ISSN

—

Svazek periodika

2009

Číslo periodika v rámci svazku

74

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

2

Strana od-do

—

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—