DETERMINING THE CONTRIBUTION OF DIFFERENT CELL TYPES IN SEMEN TO THE GLOBAL VALUES OF THE INTRACELLULAR OXIDANTS H2O2 AND PEROXYNITRITE USING FLOWCYTOMETRY

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F09%3A00009818" target="_blank" >RIV/61989592:15110/09:00009818 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

DETERMINING THE CONTRIBUTION OF DIFFERENT CELL TYPES IN SEMEN TO THE GLOBAL VALUES OF THE INTRACELLULAR OXIDANTS H2O2 AND PEROXYNITRITE USING FLOWCYTOMETRY

Popis výsledku v původním jazyce

Objective: Sperm, leukocytes and round cells contribute to total oxidants production in semen. The Chemiluminescence assay provides a global value of reactive oxygen species in semen but provides no information on the contribution of different cell types. In this study we used flowcytometry to assess the global intracellular levels of the oxidants H 2O 2 and peroxynitrite in semen and the relative contribution of each cell type, simultaneously. Design: Prospective study. Materials and Methods: Semen samples from 19 fertility men and 120 men undergoing fertility assessment were collected. After liquefaction, semen was centrifuged at 300 g for 7 min. The peletted cells were resuspended in phosphate buffered saline to achieve sperm concentration of 1.25 10 6/mL. Aliquots were incubated with DCFH-DA and DHR12 dyes to detect intracellular H 2O 2 and peroxynitrite, respectively, using flowcytometry. Gated analysis determined the relative contribution of sperm, leukocytes and round cells. Res

Název v anglickém jazyce

DETERMINING THE CONTRIBUTION OF DIFFERENT CELL TYPES IN SEMEN TO THE GLOBAL VALUES OF THE INTRACELLULAR OXIDANTS H2O2 AND PEROXYNITRITE USING FLOWCYTOMETRY

Popis výsledku anglicky

Objective: Sperm, leukocytes and round cells contribute to total oxidants production in semen. The Chemiluminescence assay provides a global value of reactive oxygen species in semen but provides no information on the contribution of different cell types. In this study we used flowcytometry to assess the global intracellular levels of the oxidants H 2O 2 and peroxynitrite in semen and the relative contribution of each cell type, simultaneously. Design: Prospective study. Materials and Methods: Semen samples from 19 fertility men and 120 men undergoing fertility assessment were collected. After liquefaction, semen was centrifuged at 300 g for 7 min. The peletted cells were resuspended in phosphate buffered saline to achieve sperm concentration of 1.25 10 6/mL. Aliquots were incubated with DCFH-DA and DHR12 dyes to detect intracellular H 2O 2 and peroxynitrite, respectively, using flowcytometry. Gated analysis determined the relative contribution of sperm, leukocytes and round cells. Res

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FK - Gynekologie a porodnictví

OECD FORD obor

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Projekt

<a href="/cs/project/1A8621" target="_blank" >1A8621: Oxidační stres v patofyziologii mužské neplodnosti</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fertility and Sterility

ISSN

0015-0282

e-ISSN

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Svazek periodika

92

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

2

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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