Capillary electrophoresis determination of thiopurine methyl transferase activity in erythrocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F09%3A33138354" target="_blank" >RIV/61989592:15110/09:33138354 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jchromb.2009.05.005" target="_blank" >http://dx.doi.org/10.1016/j.jchromb.2009.05.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jchromb.2009.05.005" target="_blank" >10.1016/j.jchromb.2009.05.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Capillary electrophoresis determination of thiopurine methyl transferase activity in erythrocytes

Popis výsledku v původním jazyce

Thiopurine S-methyltransferase (TPMT) catalyzes methylation of thiopurine drugs (e.g. 6-mercaptopurine, azathioprine). Decreased activity of TPMT is associated with hematopoietic toxicity after administration of standard doses of the drugs. We developedcapillary electrophoretic method for determination of TPMT enzyme activity in erythrocytes. Limit of quantification of the method is 1.5 micromol/L (S/N=6). The recovery of 6-methylmercaptopurine was 87.5-94.8%, imprecision value (as CV, n=10) was 1.68%(within-day) and 2.53% (between-day). Erythrocyte TPMT activities were measured in 60 healthy adult volunteers

Název v anglickém jazyce

Capillary electrophoresis determination of thiopurine methyl transferase activity in erythrocytes

Popis výsledku anglicky

Thiopurine S-methyltransferase (TPMT) catalyzes methylation of thiopurine drugs (e.g. 6-mercaptopurine, azathioprine). Decreased activity of TPMT is associated with hematopoietic toxicity after administration of standard doses of the drugs. We developedcapillary electrophoretic method for determination of TPMT enzyme activity in erythrocytes. Limit of quantification of the method is 1.5 micromol/L (S/N=6). The recovery of 6-methylmercaptopurine was 87.5-94.8%, imprecision value (as CV, n=10) was 1.68%(within-day) and 2.53% (between-day). Erythrocyte TPMT activities were measured in 60 healthy adult volunteers

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

ISSN

1570-0232

e-ISSN

—

Svazek periodika

877

Číslo periodika v rámci svazku

20-21

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

3

Strana od-do

1943-1945

Kód UT WoS článku

000267453800022

EID výsledku v databázi Scopus

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