Expression of multidrug resistance protein 1and multidrug resistance-associated protein 1in peripheral blood lymphocytes amongst children and young adults

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F13%3A33146476" target="_blank" >RIV/61989592:15110/13:33146476 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00098892:_____/13:#0000453

Výsledek na webu

<a href="http://dx.doi.org/10.5114/ceji.2013.39770" target="_blank" >http://dx.doi.org/10.5114/ceji.2013.39770</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5114/ceji.2013.39770" target="_blank" >10.5114/ceji.2013.39770</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expression of multidrug resistance protein 1and multidrug resistance-associated protein 1in peripheral blood lymphocytes amongst children and young adults

Popis výsledku v původním jazyce

Multidrug resistance protein 1 (MDR1, ABCB1 or P-glycoprotein) and multidrug resistance-associated protein 1 (MRP1, ABCC1 or LT C4 transporter) are active membrane-associated efflux transporters with broad substrate specificity. The aim of this study wasto assess the physiological expression of these proteins in the peripheral blood lymphocytes (PBLs) and selected polymorphisms in genes for these proteins in healthy children and young adults. The PBLs MDR1 expression in 41 subjects (aged from 2 monthsto 22.5 years of age) and PBLs MRP1 in 40 subjects (aged from 2 months to 22 years of age) were determined by using a flow cytometry analysis with the indirect immunofluorescence staining technique. Genotyping of rs1045642 (C3435T) and rs3213619 (T-129C)single nucleotide polymorphisms (SNPs) in ABCB1 gene and rs45511401 (G671V) SNP in ABCC1 gene was performed with TaqMan probes and by direct sequencing. We found a statistically significant dependence of PBLs MDR1 (but not PBLs MRP1) exp

Název v anglickém jazyce

Expression of multidrug resistance protein 1and multidrug resistance-associated protein 1in peripheral blood lymphocytes amongst children and young adults

Popis výsledku anglicky

Multidrug resistance protein 1 (MDR1, ABCB1 or P-glycoprotein) and multidrug resistance-associated protein 1 (MRP1, ABCC1 or LT C4 transporter) are active membrane-associated efflux transporters with broad substrate specificity. The aim of this study wasto assess the physiological expression of these proteins in the peripheral blood lymphocytes (PBLs) and selected polymorphisms in genes for these proteins in healthy children and young adults. The PBLs MDR1 expression in 41 subjects (aged from 2 monthsto 22.5 years of age) and PBLs MRP1 in 40 subjects (aged from 2 months to 22 years of age) were determined by using a flow cytometry analysis with the indirect immunofluorescence staining technique. Genotyping of rs1045642 (C3435T) and rs3213619 (T-129C)single nucleotide polymorphisms (SNPs) in ABCB1 gene and rs45511401 (G671V) SNP in ABCC1 gene was performed with TaqMan probes and by direct sequencing. We found a statistically significant dependence of PBLs MDR1 (but not PBLs MRP1) exp

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

<a href="/cs/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicína pro regionální rozvoj a lidské zdroje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Central European Journal of Immunology

ISSN

1426-3912

e-ISSN

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Svazek periodika

38

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

12

Strana od-do

518-529

Kód UT WoS článku

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EID výsledku v databázi Scopus

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