MiR-210 expression in tumor tissue and in vitro effects of its silencing in renal cell carcinoma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F13%3A33146555" target="_blank" >RIV/61989592:15110/13:33146555 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00209805:_____/13:#0000471 RIV/00216208:11150/13:10193054 RIV/00179906:_____/13:10193054 RIV/00216224:14740/13:00065569

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s13277-012-0573-2" target="_blank" >http://dx.doi.org/10.1007/s13277-012-0573-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13277-012-0573-2" target="_blank" >10.1007/s13277-012-0573-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

MiR-210 expression in tumor tissue and in vitro effects of its silencing in renal cell carcinoma

Popis výsledku v původním jazyce

Renal cell carcinoma (RCC) is the most common neoplasm of adult kidney accounting for about 3 % of adult malignancies. MicroRNAs (miRNAs) are a class of naturally occurring, short non-coding RNAs that regulate gene expression at the post-transcriptionallevel. We determined global miRNA expression profiles of RCC and parallel renal parenchyma tissues by using quantitative reverse transcriptase-polymerase chain reaction-based TaqMan low-density arrays. Afterward, we validated the difference in miR-210 expression levels on the larger group of RCC patients (35 RCC versus 10 non-tumorous parenchyma samples). Functional in vitro experiments were performed on ACHN and CAKI-2 RCC cell lines transfected with miRNA-210 inhibitor. Cell viability, apoptosis, cellcycle, scratch wound migration assay, and invasion assay (xCELLigence) were performed. We have identified original ccRCC-specific miRNA signature in clinical samples (73 miRNAs were significantly downregulated and five miRNAs upregulated

Název v anglickém jazyce

MiR-210 expression in tumor tissue and in vitro effects of its silencing in renal cell carcinoma

Popis výsledku anglicky

Renal cell carcinoma (RCC) is the most common neoplasm of adult kidney accounting for about 3 % of adult malignancies. MicroRNAs (miRNAs) are a class of naturally occurring, short non-coding RNAs that regulate gene expression at the post-transcriptionallevel. We determined global miRNA expression profiles of RCC and parallel renal parenchyma tissues by using quantitative reverse transcriptase-polymerase chain reaction-based TaqMan low-density arrays. Afterward, we validated the difference in miR-210 expression levels on the larger group of RCC patients (35 RCC versus 10 non-tumorous parenchyma samples). Functional in vitro experiments were performed on ACHN and CAKI-2 RCC cell lines transfected with miRNA-210 inhibitor. Cell viability, apoptosis, cellcycle, scratch wound migration assay, and invasion assay (xCELLigence) were performed. We have identified original ccRCC-specific miRNA signature in clinical samples (73 miRNAs were significantly downregulated and five miRNAs upregulated

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Tumor biology

ISSN

1010-4283

e-ISSN

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Svazek periodika

34

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

481-491

Kód UT WoS článku

000313875400056

EID výsledku v databázi Scopus

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