Silymarin Constituent 2,3-Dehydrosilybin Triggers Reserpine-Sensitive Positive Inotropic Effect in Perfused Rat Heart
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A33154543" target="_blank" >RIV/61989592:15110/15:33154543 - isvavai.cz</a>
Výsledek na webu
<a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139208" target="_blank" >http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139208</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0139208" target="_blank" >10.1371/journal.pone.0139208</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Silymarin Constituent 2,3-Dehydrosilybin Triggers Reserpine-Sensitive Positive Inotropic Effect in Perfused Rat Heart
Popis výsledku v původním jazyce
2,3-dehydrosilybin (DHS) is a minor flavonolignan component of Silybum marianum seed extract known for its hepatoprotective activity. Recently we identified DHS as a potentially cardioprotective substance during hypoxia/reoxygenation in isolated neonatalrat cardiomyocytes. This is the first report of positive inotropic effect of DHS on perfused adult rat heart. When applied to perfused adult rat heart, DHS caused a dose-dependent inotropic effect resembling that of catecholamines. The effect was apparent with DHS concentration as low as 10 nM. Suspecting direct interaction with ?-adrenergic receptors, we tested whether DHS can trigger ? agonist-dependent gene transcription in a model cell line. While DHS alone was unable to trigger ? agonist-dependentgene transcription, it enhanced the effect of isoproterenol, a known unspecific ? agonist. Further tests confirmed that DHS could not induce cAMP accumulation in isolated neonatal rat cardiomyocytes even though high concentrations (10 ?M
Název v anglickém jazyce
Silymarin Constituent 2,3-Dehydrosilybin Triggers Reserpine-Sensitive Positive Inotropic Effect in Perfused Rat Heart
Popis výsledku anglicky
2,3-dehydrosilybin (DHS) is a minor flavonolignan component of Silybum marianum seed extract known for its hepatoprotective activity. Recently we identified DHS as a potentially cardioprotective substance during hypoxia/reoxygenation in isolated neonatalrat cardiomyocytes. This is the first report of positive inotropic effect of DHS on perfused adult rat heart. When applied to perfused adult rat heart, DHS caused a dose-dependent inotropic effect resembling that of catecholamines. The effect was apparent with DHS concentration as low as 10 nM. Suspecting direct interaction with ?-adrenergic receptors, we tested whether DHS can trigger ? agonist-dependent gene transcription in a model cell line. While DHS alone was unable to trigger ? agonist-dependentgene transcription, it enhanced the effect of isoproterenol, a known unspecific ? agonist. Further tests confirmed that DHS could not induce cAMP accumulation in isolated neonatal rat cardiomyocytes even though high concentrations (10 ?M
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
PLoS One
ISSN
1932-6203
e-ISSN
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Svazek periodika
10
Číslo periodika v rámci svazku
9
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
15
Strana od-do
"e0139208-1"-"e0139208-15"
Kód UT WoS článku
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EID výsledku v databázi Scopus
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