Simultaneous determination of mushroom toxins ?-amanitin, ?-amanitin and muscarine in human urine by solid-phase extraction and ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A33155700" target="_blank" >RIV/61989592:15110/15:33155700 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00098892:_____/15:#0000975

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S037907381500153" target="_blank" >http://www.sciencedirect.com/science/article/pii/S037907381500153</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.forsciint.2015.04.007" target="_blank" >10.1016/j.forsciint.2015.04.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Simultaneous determination of mushroom toxins ?-amanitin, ?-amanitin and muscarine in human urine by solid-phase extraction and ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry

Popis výsledku v původním jazyce

This paper presents a method for the simultaneous determination of ?-amanitin, ?-amanitin and muscarine in human urine by solid-phase extraction (SPE) and ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry. The method can be used for a diagnostics of mushroom poisonings. Different SPE cartridges were tested for sample preparation, namely hydrophilic modified reversed-phase (Oasis HLB) and polymeric weak cation phase (Strata XCW).The latter gave better results and therefore it was chosen for the subsequent method optimization and partial validation. In the course of validation, limits of detection, linearity, intraday and interday precisions and recoveries were evaluated. The obtained LOD values of ?-amanitin and ?-amanitin were1 ng/mL and of muscarine 0.09 ng/mL. The intraday and interday precisions of human urine spiked with ?-amanitin (10 ng/mL), ?-amanitin (10 ng/mL) and muscarine (1 ng/mL) ranged from 6% to 10% and from 7% to 13%,

Název v anglickém jazyce

Simultaneous determination of mushroom toxins ?-amanitin, ?-amanitin and muscarine in human urine by solid-phase extraction and ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry

Popis výsledku anglicky

This paper presents a method for the simultaneous determination of ?-amanitin, ?-amanitin and muscarine in human urine by solid-phase extraction (SPE) and ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry. The method can be used for a diagnostics of mushroom poisonings. Different SPE cartridges were tested for sample preparation, namely hydrophilic modified reversed-phase (Oasis HLB) and polymeric weak cation phase (Strata XCW).The latter gave better results and therefore it was chosen for the subsequent method optimization and partial validation. In the course of validation, limits of detection, linearity, intraday and interday precisions and recoveries were evaluated. The obtained LOD values of ?-amanitin and ?-amanitin were1 ng/mL and of muscarine 0.09 ng/mL. The intraday and interday precisions of human urine spiked with ?-amanitin (10 ng/mL), ?-amanitin (10 ng/mL) and muscarine (1 ng/mL) ranged from 6% to 10% and from 7% to 13%,

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Forensic Science International

ISSN

0379-0738

e-ISSN

—

Svazek periodika

2015

Číslo periodika v rámci svazku

251

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

209-213

Kód UT WoS článku

000354125900031

EID výsledku v databázi Scopus

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