Determination of muscarine in human urine by electrospray liquid chromatographic-mass spectrometric

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F11%3A00102032" target="_blank" >RIV/00843989:_____/11:00102032 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15110/11:33118206 RIV/61989592:15310/11:33118206

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jchromb.2011.07.009" target="_blank" >http://dx.doi.org/10.1016/j.jchromb.2011.07.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jchromb.2011.07.009" target="_blank" >10.1016/j.jchromb.2011.07.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of muscarine in human urine by electrospray liquid chromatographic-mass spectrometric

Popis výsledku v původním jazyce

A liquid chromatography-mass spectrometry based method for determination of muscarine in human urine was developed and validated. The method involved a solid phase extraction of muscarine from urine using Strata X-CW column. Separation of muscarine was achieved within 16.0 min on a reversed phase Gemini C18 analytical column (150 mm x 2.0 mm id., 5 mu m) with a mobile phase consisted of aqueous 8 mmol/L heptafluorobutyric acid and acetonitrile in a gradient mode. Mass spectrometric detection was performed at m/z 174 and m/z 216 for muscarine and acetylmuscarine (internal standard), respectively. The linearity was satisfactory with a coefficient of determination (R(2)) 0.9993 at concentration range from 0.3 ng/mL to 2.0 mu g/mL. LOD and LOQ for muscarine was 0.09 ng/mL and 0.3 ng/mL, respectively. The found out recoveries of muscarine were 96% or 95% for concentration 0.3 ng/mL and 0.2 mu g/mL or 2.0 mu g/mL, respectively. The precision in the intra-assay-study varied from 0.48% to 1.39

Název v anglickém jazyce

Determination of muscarine in human urine by electrospray liquid chromatographic-mass spectrometric

Popis výsledku anglicky

A liquid chromatography-mass spectrometry based method for determination of muscarine in human urine was developed and validated. The method involved a solid phase extraction of muscarine from urine using Strata X-CW column. Separation of muscarine was achieved within 16.0 min on a reversed phase Gemini C18 analytical column (150 mm x 2.0 mm id., 5 mu m) with a mobile phase consisted of aqueous 8 mmol/L heptafluorobutyric acid and acetonitrile in a gradient mode. Mass spectrometric detection was performed at m/z 174 and m/z 216 for muscarine and acetylmuscarine (internal standard), respectively. The linearity was satisfactory with a coefficient of determination (R(2)) 0.9993 at concentration range from 0.3 ng/mL to 2.0 mu g/mL. LOD and LOQ for muscarine was 0.09 ng/mL and 0.3 ng/mL, respectively. The found out recoveries of muscarine were 96% or 95% for concentration 0.3 ng/mL and 0.2 mu g/mL or 2.0 mu g/mL, respectively. The precision in the intra-assay-study varied from 0.48% to 1.39

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

—

Projekt

<a href="/cs/project/NS10269" target="_blank" >NS10269: Objektivní analytická metoda k diagnostice intoxikací muchomůrkami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of chromatography B

ISSN

1570-0232

e-ISSN

—

Svazek periodika

879

Číslo periodika v rámci svazku

25

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

2549-2553

Kód UT WoS článku

000294982600011

EID výsledku v databázi Scopus

—