Imaging Flow Cytometry as a Sensitive Tool to Detect Low-Dose-Induced DNA Damage by Analyzing 53BP1 and gamma H2AX Foci in Human Lymphocytes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A33158244" target="_blank" >RIV/61989592:15110/15:33158244 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1002/cyto.a.22731" target="_blank" >http://dx.doi.org/10.1002/cyto.a.22731</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cyto.a.22731" target="_blank" >10.1002/cyto.a.22731</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Imaging Flow Cytometry as a Sensitive Tool to Detect Low-Dose-Induced DNA Damage by Analyzing 53BP1 and gamma H2AX Foci in Human Lymphocytes
Popis výsledku v původním jazyce
Ionizing radiation induced foci (IRIF) are considered the most sensitive indicator for DNA double-strand break (DSB) detection. Monitoring DSB induction by low doses of ionizing radiation is important due to the increasing exposure in the general population. gamma H2AX and 53BP1 are commonly used molecular markers for in situ IRIF assessment. Imaging flow cytometry (IFC) via ImageStream system provides a new opportunity in this field. We analyzed the formation of 53BP1, gamma H2AX foci and their co-localization induced by gamma-rays (2, 5, 10, 50, 200 cGy) in human lymphocytes using ImageStream and the automated microscopic system Metafer. We observed very similar sensitivity of both systems for the detection of endogenous and low-dose-induced IRIF. Statistically significant induction of gamma H2AX foci was found at doses of 2 and 10 cGy using ImageStream and Metafer, respectively. Statistically significant induction of 53BP1 foci was evident at doses }= 5 cGy when analyzed by IFC. Ana
Název v anglickém jazyce
Imaging Flow Cytometry as a Sensitive Tool to Detect Low-Dose-Induced DNA Damage by Analyzing 53BP1 and gamma H2AX Foci in Human Lymphocytes
Popis výsledku anglicky
Ionizing radiation induced foci (IRIF) are considered the most sensitive indicator for DNA double-strand break (DSB) detection. Monitoring DSB induction by low doses of ionizing radiation is important due to the increasing exposure in the general population. gamma H2AX and 53BP1 are commonly used molecular markers for in situ IRIF assessment. Imaging flow cytometry (IFC) via ImageStream system provides a new opportunity in this field. We analyzed the formation of 53BP1, gamma H2AX foci and their co-localization induced by gamma-rays (2, 5, 10, 50, 200 cGy) in human lymphocytes using ImageStream and the automated microscopic system Metafer. We observed very similar sensitivity of both systems for the detection of endogenous and low-dose-induced IRIF. Statistically significant induction of gamma H2AX foci was found at doses of 2 and 10 cGy using ImageStream and Metafer, respectively. Statistically significant induction of 53BP1 foci was evident at doses }= 5 cGy when analyzed by IFC. Ana
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Cytometry. Part A
ISSN
1552-4922
e-ISSN
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Svazek periodika
87A
Číslo periodika v rámci svazku
12
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
9
Strana od-do
1070-1078
Kód UT WoS článku
000367670800003
EID výsledku v databázi Scopus
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