Imaging of Pseudomonas aeruginosa infection with Ga-68 labelled pyoverdine for positron emission tomography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F18%3A73590728" target="_blank" >RIV/61989592:15110/18:73590728 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/18:00496187 RIV/61989592:15310/18:73590728

Výsledek na webu

<a href="https://www.ncbi.nlm.nih.gov/pubmed/?term=Imaging+of+Pseudomonas+aeruginosa+infection+with+Ga-68+labelled+pyoverdine+for+positron+emission+tomography" target="_blank" >https://www.ncbi.nlm.nih.gov/pubmed/?term=Imaging+of+Pseudomonas+aeruginosa+infection+with+Ga-68+labelled+pyoverdine+for+positron+emission+tomography</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-018-33895-w" target="_blank" >10.1038/s41598-018-33895-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Imaging of Pseudomonas aeruginosa infection with Ga-68 labelled pyoverdine for positron emission tomography

Popis výsledku v původním jazyce

Pseudomonas aeruginosa is an increasingly prevalent opportunistic pathogen that causes a variety of life-threatening nosocomial infections. Novel strategies for the development of new antibacterial treatments as well as diagnostic tools are needed. One of the novel diagnostic strategies for the detection of infection could be the utilization of siderophores. Siderophores are low-molecular-weight chelators produced by microbes to scavenge essential iron. Replacing iron in siderophores by suitable radiometals, such as Ga-68 for positron emission tomography (PET) imaging, opens approaches for targeted imaging of infection. Here we report on pyoverdine PAO1 (PVD-PAO1), a siderophore produced by P. aeruginosa, labelled with Ga-68 for specific imaging of Pseudomonas infections. PVD-PAO1 was labelled with Ga-68 with high radiochemical purity. The resulting complex showed hydrophilic properties, low protein binding and high stability in human serum. In vitro uptake of Ga-68-PVD-PAO1 was highly dependent on the type of microbial culture. In normal mice Ga-68-PVD-PAO1 showed rapid pharmacokinetics with urinary excretion. PET imaging in infected animals displayed specific accumulation of Ga-68-PVD-PAO1 in infected tissues and better distribution than clinically used F-18-fluorodeoxyglucose (F-18-FDG) and Ga-68-citrate. Ga-68 labelled pyoverdine PAO1 seems to be a promising agent for imaging of P. aeruginosa infections by means of PET.

Název v anglickém jazyce

Imaging of Pseudomonas aeruginosa infection with Ga-68 labelled pyoverdine for positron emission tomography

Popis výsledku anglicky

Pseudomonas aeruginosa is an increasingly prevalent opportunistic pathogen that causes a variety of life-threatening nosocomial infections. Novel strategies for the development of new antibacterial treatments as well as diagnostic tools are needed. One of the novel diagnostic strategies for the detection of infection could be the utilization of siderophores. Siderophores are low-molecular-weight chelators produced by microbes to scavenge essential iron. Replacing iron in siderophores by suitable radiometals, such as Ga-68 for positron emission tomography (PET) imaging, opens approaches for targeted imaging of infection. Here we report on pyoverdine PAO1 (PVD-PAO1), a siderophore produced by P. aeruginosa, labelled with Ga-68 for specific imaging of Pseudomonas infections. PVD-PAO1 was labelled with Ga-68 with high radiochemical purity. The resulting complex showed hydrophilic properties, low protein binding and high stability in human serum. In vitro uptake of Ga-68-PVD-PAO1 was highly dependent on the type of microbial culture. In normal mice Ga-68-PVD-PAO1 showed rapid pharmacokinetics with urinary excretion. PET imaging in infected animals displayed specific accumulation of Ga-68-PVD-PAO1 in infected tissues and better distribution than clinically used F-18-fluorodeoxyglucose (F-18-FDG) and Ga-68-citrate. Ga-68 labelled pyoverdine PAO1 seems to be a promising agent for imaging of P. aeruginosa infections by means of PET.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10700 - Other natural sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

OCT 24 2018

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

"nestránkováno"

Kód UT WoS článku

000448109000023

EID výsledku v databázi Scopus

2-s2.0-85055459467