Effect of the amino acid substitution in the DNA-binding domain of the Fur regulator on production of pyoverdine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00426109" target="_blank" >RIV/61388971:_____/13:00426109 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s12223-012-0210-x" target="_blank" >http://dx.doi.org/10.1007/s12223-012-0210-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-012-0210-x" target="_blank" >10.1007/s12223-012-0210-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of the amino acid substitution in the DNA-binding domain of the Fur regulator on production of pyoverdine

Popis výsledku v původním jazyce

The ferric uptake regulator gene (fur), its promoter region and Fur box of pvdS gene involved in siderophore-mediated iron uptake system were sequenced in the parent strain Pseudomonas aeruginosa PAO1 and in the fur mutant FPA121 derived from the strainPAO1. We identified the gene fur(179) bearing a novel, single-point mutation that changed the amino acid residue Gln60Pro in the DNA-binding domain of the Fur protein. The synthesis of pyoverdine was studied in cultures of the strains PAO1 and FPA121 grown in iron-deplete and iron-replete (60 mu mol/L FeIII) medium. The amino acid replacement in the regulatory Fur protein is responsible for the overproduction of pyoverdine in iron-deplete and iron-replete medium. No mutation was identified in the Fur box of the gene pvdS.

Název v anglickém jazyce

Effect of the amino acid substitution in the DNA-binding domain of the Fur regulator on production of pyoverdine

Popis výsledku anglicky

The ferric uptake regulator gene (fur), its promoter region and Fur box of pvdS gene involved in siderophore-mediated iron uptake system were sequenced in the parent strain Pseudomonas aeruginosa PAO1 and in the fur mutant FPA121 derived from the strainPAO1. We identified the gene fur(179) bearing a novel, single-point mutation that changed the amino acid residue Gln60Pro in the DNA-binding domain of the Fur protein. The synthesis of pyoverdine was studied in cultures of the strains PAO1 and FPA121 grown in iron-deplete and iron-replete (60 mu mol/L FeIII) medium. The amino acid replacement in the regulatory Fur protein is responsible for the overproduction of pyoverdine in iron-deplete and iron-replete medium. No mutation was identified in the Fur box of the gene pvdS.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/KJB500200703" target="_blank" >KJB500200703: Charakterizace Fur fenotypu mutantů Pseudomonas aeruginosa: nadprodukce komponent transportního systému pro FeIII.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

—

Svazek periodika

58

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

311-317

Kód UT WoS článku

000322712700007

EID výsledku v databázi Scopus

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