Mechanism of liposome formation by microfluidic mixing: the concept based on lipid bilayer fragments vesiculation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F18%3A73591347" target="_blank" >RIV/61989592:15110/18:73591347 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Mechanism of liposome formation by microfluidic mixing: the concept based on lipid bilayer fragments vesiculation

Popis výsledku v původním jazyce

Liposomes are known for their biocompatible and encapsulation properties and are the most successful drug-carrier system approved by FDA. Recently new preparation technique based on nanofluidic mixing has been demonstrated a using microfluidic platform with characteristic mixing profile. The instrument NanoAssemblrTM (Precission Nanosystems, Canada) was used to prepare nanoliposomes containing metallochelating lipid 18:0 PE DTPA (Gd) to improve contrast in cryoTEM and resolve individual parts of membrane layers by cryoTEM. The size distribution was analyzed by DLS (Zetasizer Nano ZSP, Malvern Instruments, UK) and NTA (NanoSight NS500, Malvern Instruments, UK), liposomeswere (114±0.3) nm in diameter with low polydispersity 0.04±0.02. By NMR (Bruker-BioSpec 94/30 USR, Bruker, GE) measurement, the use of our Gd-liposomes as theranostic has been proven. Relaxivity was much higher, around 40 mM−1·s−1 compared to common contrast agent for MRI. Using ICP-OES (ULTIMA 2, Horiba Jobin Yvon, FR) analytical measurement, the precise concentration of gadolinium was terminated to (9.30±1.49) mg·l−1. This concentration is suitable for potential in vivoexperiments. Liposomes can be usedas targeted diagnostic agents and will contain encapsulated APIs for therapeutic use. An important observation was the detection of lipid membrane fragments in liposomes. This work also experimentally describes the concept of liposome formulation from a lipid bilayer fragments. This new concept of was proven by modern analytical methods including cryotransmission electron microscopy technique,which allows us to observe single liposomes containing fragment and also double liposomes prior to conversion.

Název v anglickém jazyce

Mechanism of liposome formation by microfluidic mixing: the concept based on lipid bilayer fragments vesiculation

Popis výsledku anglicky

Liposomes are known for their biocompatible and encapsulation properties and are the most successful drug-carrier system approved by FDA. Recently new preparation technique based on nanofluidic mixing has been demonstrated a using microfluidic platform with characteristic mixing profile. The instrument NanoAssemblrTM (Precission Nanosystems, Canada) was used to prepare nanoliposomes containing metallochelating lipid 18:0 PE DTPA (Gd) to improve contrast in cryoTEM and resolve individual parts of membrane layers by cryoTEM. The size distribution was analyzed by DLS (Zetasizer Nano ZSP, Malvern Instruments, UK) and NTA (NanoSight NS500, Malvern Instruments, UK), liposomeswere (114±0.3) nm in diameter with low polydispersity 0.04±0.02. By NMR (Bruker-BioSpec 94/30 USR, Bruker, GE) measurement, the use of our Gd-liposomes as theranostic has been proven. Relaxivity was much higher, around 40 mM−1·s−1 compared to common contrast agent for MRI. Using ICP-OES (ULTIMA 2, Horiba Jobin Yvon, FR) analytical measurement, the precise concentration of gadolinium was terminated to (9.30±1.49) mg·l−1. This concentration is suitable for potential in vivoexperiments. Liposomes can be usedas targeted diagnostic agents and will contain encapsulated APIs for therapeutic use. An important observation was the detection of lipid membrane fragments in liposomes. This work also experimentally describes the concept of liposome formulation from a lipid bilayer fragments. This new concept of was proven by modern analytical methods including cryotransmission electron microscopy technique,which allows us to observe single liposomes containing fragment and also double liposomes prior to conversion.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/NV15-32198A" target="_blank" >NV15-32198A: Příprava rekombinantních mimotopů indukujících neutralizační protilátky proti HIV-1 gp120 glykoproteinu pomocí technologie vysokoafinitních ligandů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

11th International Conference Drug Delivery Systems Nanotechnology for Healthcare: Progress in Recombinant Vaccines, Molecular Adjuvants, Modern Drug Delivery Systems and Cell Therapy

ISBN

978-80-907074-6-7

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

9

Strana od-do

89-97

Název nakladatele

nakladatelství Machovský

Místo vydání

Olomouc

Místo konání akce

Telč

Datum konání akce

5. 6. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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