Preparation of nanoliposomes by microfuidic mixing in herring-bone channel and the role of membrane fluidity in liposomes formation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73602953" target="_blank" >RIV/61989592:15110/20:73602953 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-020-62500-2" target="_blank" >https://www.nature.com/articles/s41598-020-62500-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-020-62500-2" target="_blank" >10.1038/s41598-020-62500-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Preparation of nanoliposomes by microfuidic mixing in herring-bone channel and the role of membrane fluidity in liposomes formation

Popis výsledku v původním jazyce

Introduction of microfuidic mixing technique opens a new door for preparation of the liposomes and lipid-based nanoparticles by on-chip technologies that are applicable in a laboratory and industrial scale. This study demonstrates the role of phospholipid bilayer fragment as the key intermediate in the mechanism of liposome formation by microfuidic mixing in the channel with “herring-bone” geometry used with the instrument NanoAssemblr. The fuidity of the lipid bilayer expressed as fuorescence anisotropy of the probe N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl) was found to be the basic parameter afecting the fnal size of formed liposomes prepared by microfuidic mixing of an ethanol solution of lipids and water phase. Both saturated and unsaturated lipids together with various content of cholesterol were used for liposome preparation and it was demonstrated, that an increase in fuidity results in a decrease of liposome size as analyzed by DLS. Gadolinium chelating lipids were used to visualize the fne structure of liposomes and bilayer fragments by CryoTEM. Experimental data and theoretical calculations are in good accordance with the theory of lipid disc micelle vesiculation.

Název v anglickém jazyce

Preparation of nanoliposomes by microfuidic mixing in herring-bone channel and the role of membrane fluidity in liposomes formation

Popis výsledku anglicky

Introduction of microfuidic mixing technique opens a new door for preparation of the liposomes and lipid-based nanoparticles by on-chip technologies that are applicable in a laboratory and industrial scale. This study demonstrates the role of phospholipid bilayer fragment as the key intermediate in the mechanism of liposome formation by microfuidic mixing in the channel with “herring-bone” geometry used with the instrument NanoAssemblr. The fuidity of the lipid bilayer expressed as fuorescence anisotropy of the probe N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl) was found to be the basic parameter afecting the fnal size of formed liposomes prepared by microfuidic mixing of an ethanol solution of lipids and water phase. Both saturated and unsaturated lipids together with various content of cholesterol were used for liposome preparation and it was demonstrated, that an increase in fuidity results in a decrease of liposome size as analyzed by DLS. Gadolinium chelating lipids were used to visualize the fne structure of liposomes and bilayer fragments by CryoTEM. Experimental data and theoretical calculations are in good accordance with the theory of lipid disc micelle vesiculation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30404 - Biomaterials (as related to medical implants, devices, sensors)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

"'5595(1)'"-"'5595(11)'"

Kód UT WoS článku

000546050500001

EID výsledku v databázi Scopus

2-s2.0-85082509909