Observation of lubrication mechanisms in knee replacement: A pilot study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F19%3A73593097" target="_blank" >RIV/61989592:15110/19:73593097 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26210/19:PU131064

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2352573818300416#" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2352573818300416#</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biotri.2019.02.001" target="_blank" >10.1016/j.biotri.2019.02.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Observation of lubrication mechanisms in knee replacement: A pilot study

Popis výsledku v původním jazyce

The present study introduces a unique experimental approach for in situ observation of lubricant film formation in knee joint replacements. A knee joint simulator was designed and equipped with optical module based on fluorescent optical method for film thickness observation. The contact between the femoral knee metal implant and real-shaped polymer insert mimicking actual contact nature is observed. The shape of the polymer insert was fabricated with respect to the shape of original polyethylene insert to ensure corresponding contact conformity. Simple solutions of albumin and γ-globulin proteins as well as its mixture were used while the film thickness was studied as a function of time considering simplified flexion/extension motion with variable load over the cycle. Adequate fluorescent markers were employed enabling to observe one particular protein during each measurement. The results showed a clear importance of the interaction of proteins since the mixtures showed different results compared to simple solutions. Especially considering albumin protein, its behaviour was substantially affected by adding γ-globulin. Moreover, a satisfactory compliance with previous findings related to hip joint lubrication in terms of the behaviour of both proteins was found. Finally, the motivation for future experimental work is highlighted.

Název v anglickém jazyce

Observation of lubrication mechanisms in knee replacement: A pilot study

Popis výsledku anglicky

The present study introduces a unique experimental approach for in situ observation of lubricant film formation in knee joint replacements. A knee joint simulator was designed and equipped with optical module based on fluorescent optical method for film thickness observation. The contact between the femoral knee metal implant and real-shaped polymer insert mimicking actual contact nature is observed. The shape of the polymer insert was fabricated with respect to the shape of original polyethylene insert to ensure corresponding contact conformity. Simple solutions of albumin and γ-globulin proteins as well as its mixture were used while the film thickness was studied as a function of time considering simplified flexion/extension motion with variable load over the cycle. Adequate fluorescent markers were employed enabling to observe one particular protein during each measurement. The results showed a clear importance of the interaction of proteins since the mixtures showed different results compared to simple solutions. Especially considering albumin protein, its behaviour was substantially affected by adding γ-globulin. Moreover, a satisfactory compliance with previous findings related to hip joint lubrication in terms of the behaviour of both proteins was found. Finally, the motivation for future experimental work is highlighted.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

30404 - Biomaterials (as related to medical implants, devices, sensors)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biotribology

ISSN

2352-5738

e-ISSN

—

Svazek periodika

71

Číslo periodika v rámci svazku

February

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

1-7

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85061051598