Antifungal susceptibility profiles of rare ascomycetous yeasts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F19%3A73605656" target="_blank" >RIV/61989592:15110/19:73605656 - isvavai.cz</a>

Výsledek na webu

<a href="https://academic.oup.com/jac/article/74/9/2649/5519369" target="_blank" >https://academic.oup.com/jac/article/74/9/2649/5519369</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Antifungal susceptibility profiles of rare ascomycetous yeasts

Popis výsledku v původním jazyce

Objectives: To generate antifungal susceptibility patterns for Trichomonascus ciferrii (Candida ciferrii), Candida inconspicua (Torulopsis inconspicua) and Diutina rugosa species complex (Candida rugosa species complex), and to provide key parameters such as MIC50, MIC90 and tentative epidemiological cut-off values (TECOFFs). Methods: Our strain set included isolates of clinical origin: C. inconspicua (n = 168), D. rugosa species complex (n = 90) [Candida pararugosa (n = 60), D. rugosa (n = 26) and Candida mesorugosa (n = 4)], Pichia norvegensis (Candida norvegensis) (n = 15) and T. ciferrii (n = 8). Identification was performed by MALDI-TOF MS or internal transcribed spacer sequencing. Antifungal susceptibility patterns were generated for azoles, echinocandins and amphotericin B using commercial Etest and the EUCAST broth microdilution method v7.3.1. Essential agreement (EA) was calculated for Etest and EUCAST. Results: C. inconspicua, C. pararugosa and P. norvegensis showed elevated azole MICs (MIC50 &gt;= 0.06 mg/L), and D. rugosa and C. pararugosa elevated echinocandin MICs (MIC50 &gt;= 0.06 mg/L). EA between methods was generally low (&lt;90%); EA averaged 77.45%. TECOFFs were suggested for C. inconspicua and D. rugosa species complex. Conclusions: Rare yeast species tested shared high fluconazole MICs. D. rugosa species complex displayed high echinocandin MICs, while C. inconspicua and P. norvegensis were found to have high azole MICs. Overall, the agreement between EUCAST and Etest was poor and therefore MIC values generated with Etest cannot be directly compared with EUCAST results.

Název v anglickém jazyce

Antifungal susceptibility profiles of rare ascomycetous yeasts

Popis výsledku anglicky

Objectives: To generate antifungal susceptibility patterns for Trichomonascus ciferrii (Candida ciferrii), Candida inconspicua (Torulopsis inconspicua) and Diutina rugosa species complex (Candida rugosa species complex), and to provide key parameters such as MIC50, MIC90 and tentative epidemiological cut-off values (TECOFFs). Methods: Our strain set included isolates of clinical origin: C. inconspicua (n = 168), D. rugosa species complex (n = 90) [Candida pararugosa (n = 60), D. rugosa (n = 26) and Candida mesorugosa (n = 4)], Pichia norvegensis (Candida norvegensis) (n = 15) and T. ciferrii (n = 8). Identification was performed by MALDI-TOF MS or internal transcribed spacer sequencing. Antifungal susceptibility patterns were generated for azoles, echinocandins and amphotericin B using commercial Etest and the EUCAST broth microdilution method v7.3.1. Essential agreement (EA) was calculated for Etest and EUCAST. Results: C. inconspicua, C. pararugosa and P. norvegensis showed elevated azole MICs (MIC50 &gt;= 0.06 mg/L), and D. rugosa and C. pararugosa elevated echinocandin MICs (MIC50 &gt;= 0.06 mg/L). EA between methods was generally low (&lt;90%); EA averaged 77.45%. TECOFFs were suggested for C. inconspicua and D. rugosa species complex. Conclusions: Rare yeast species tested shared high fluconazole MICs. D. rugosa species complex displayed high echinocandin MICs, while C. inconspicua and P. norvegensis were found to have high azole MICs. Overall, the agreement between EUCAST and Etest was poor and therefore MIC values generated with Etest cannot be directly compared with EUCAST results.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

ISSN

0305-7453

e-ISSN

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Svazek periodika

74

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

2649-2656

Kód UT WoS článku

000487303300021

EID výsledku v databázi Scopus

2-s2.0-85072057511