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Method for the determination of broth dilution minimum inhibitory cencentrations of antifungal agents for yeasts

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73605690" target="_blank" >RIV/61989592:15110/20:73605690 - isvavai.cz</a>

  • Výsledek na webu

    <a href="https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/AFST/Files/EUCAST_E_Def_7.3.2_Yeast_testing_definitive_revised_2020.pdf" target="_blank" >https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/AFST/Files/EUCAST_E_Def_7.3.2_Yeast_testing_definitive_revised_2020.pdf</a>

  • DOI - Digital Object Identifier

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Method for the determination of broth dilution minimum inhibitory cencentrations of antifungal agents for yeasts

  • Popis výsledku v původním jazyce

    Antifungal susceptibility tests are performed on fungi causing disease especially if the infection is invasive, relapsing or failing therapy, when inherent or acquired resistance is a possibility or when susceptibility cannot reliably be predicted from the species identification alone. Antifungal susceptibility testing (AFST) is also important for resistance surveillance, epidemiological studies and for comparison of the in vitro activity of new and existing agents. Dilution methods are used to establish the minimum inhibitory concentrations (MICs) of antimicrobial agents: They are the reference methods for antimicrobial susceptibility testing, and are mainly used: to establish the activity of new antifungal agents, to confirm the susceptibility of organisms that give equivocal results in other test formats (such as commercial tests), and to determine the susceptibility of organisms where other test formats may be unreliable or not yet validated. In dilution tests, fungi are evaluated for their ability to produce sufficient growth in microdilution plate wells of broth culture media containing serial dilutions of the antimicrobial agents (broth microdilution). The antifungal MIC is defined as the lowest concentration, recorded in mg/L, of an agent that inhibits the growth of a fungus to a predefined degree (e.g. 50%, 90%, or complete growth inhibition). The MIC informs about the susceptibility or resistance of the organism to the antifungal agent, which can help in treatment decisions. This 7.3.1 method update is intended for testing the susceptibility of medically important yeasts (primarily Candida and Cryptococcus spp). The first version of the standard was published as a discussion document (7.1) in 2003 [1] and the definitive document in 2008 [2]. The second version contained additional information concerning solvent for caspofungin, micafungin and fluconazole, shelf-life of plates containing the echinocandins, testing of Cryptococcus and reference MIC ranges for quality control strains and anidulafungin [3]. This third version has been harmonised in wording and formatting to align with the mould 9.3.2 definitive document, the revised definition of the I category has been introduced and MIC ranges for quality control strains have been removed (this has been done acknowledging the new separate document summarising all antifungal MIC ranges for quality control strains (available on the EUCAST website http://www.EUCAST.org and in order to avoid a need for method document updating whenever a new QC range is established). Furthermore, the section concerning the preparation and calibration of the spectrophotometer has been modified in the current document

  • Název v anglickém jazyce

    Method for the determination of broth dilution minimum inhibitory cencentrations of antifungal agents for yeasts

  • Popis výsledku anglicky

    Antifungal susceptibility tests are performed on fungi causing disease especially if the infection is invasive, relapsing or failing therapy, when inherent or acquired resistance is a possibility or when susceptibility cannot reliably be predicted from the species identification alone. Antifungal susceptibility testing (AFST) is also important for resistance surveillance, epidemiological studies and for comparison of the in vitro activity of new and existing agents. Dilution methods are used to establish the minimum inhibitory concentrations (MICs) of antimicrobial agents: They are the reference methods for antimicrobial susceptibility testing, and are mainly used: to establish the activity of new antifungal agents, to confirm the susceptibility of organisms that give equivocal results in other test formats (such as commercial tests), and to determine the susceptibility of organisms where other test formats may be unreliable or not yet validated. In dilution tests, fungi are evaluated for their ability to produce sufficient growth in microdilution plate wells of broth culture media containing serial dilutions of the antimicrobial agents (broth microdilution). The antifungal MIC is defined as the lowest concentration, recorded in mg/L, of an agent that inhibits the growth of a fungus to a predefined degree (e.g. 50%, 90%, or complete growth inhibition). The MIC informs about the susceptibility or resistance of the organism to the antifungal agent, which can help in treatment decisions. This 7.3.1 method update is intended for testing the susceptibility of medically important yeasts (primarily Candida and Cryptococcus spp). The first version of the standard was published as a discussion document (7.1) in 2003 [1] and the definitive document in 2008 [2]. The second version contained additional information concerning solvent for caspofungin, micafungin and fluconazole, shelf-life of plates containing the echinocandins, testing of Cryptococcus and reference MIC ranges for quality control strains and anidulafungin [3]. This third version has been harmonised in wording and formatting to align with the mould 9.3.2 definitive document, the revised definition of the I category has been introduced and MIC ranges for quality control strains have been removed (this has been done acknowledging the new separate document summarising all antifungal MIC ranges for quality control strains (available on the EUCAST website http://www.EUCAST.org and in order to avoid a need for method document updating whenever a new QC range is established). Furthermore, the section concerning the preparation and calibration of the spectrophotometer has been modified in the current document

Klasifikace

  • Druh

    N<sub>metS</sub> - Metodiky schválené orgánem státní správy

  • CEP obor

  • OECD FORD obor

    10612 - Mycology

Návaznosti výsledku

  • Projekt

  • Návaznosti

    N - Vyzkumna aktivita podporovana z neverejnych zdroju

Ostatní

  • Rok uplatnění

    2020

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Interní identifikační kód produktu

    Kvasinkovité mikroorganismy

  • Číslo předpisu

    E.DEF 7.3.2

  • Technické parametry

    E.DEF 7.3.2, EUCAST AFST

  • Ekonomické parametry

    nová verze Evropského konsenzu testování citlivosti k ATB

  • Označení certifikačního orgánu

    Eucast

  • Datum certifikace

  • Způsoby využití výsledku

    C - Výsledek je využíván bez omezení okruhu uživatelů