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Method for the determination of broth dilution minimum inhibitory concetrations of antifungal agents for conidia forming moulds

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73605691" target="_blank" >RIV/61989592:15110/20:73605691 - isvavai.cz</a>

  • Výsledek na webu

    <a href="https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/AFST/Files/EUCAST_E_Def_9.3.2_Mould_testing_definitive_revised_2020.pdf" target="_blank" >https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/AFST/Files/EUCAST_E_Def_9.3.2_Mould_testing_definitive_revised_2020.pdf</a>

  • DOI - Digital Object Identifier

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Method for the determination of broth dilution minimum inhibitory concetrations of antifungal agents for conidia forming moulds

  • Popis výsledku v původním jazyce

    Antifungal susceptibility tests are performed on fungi causing disease especially when infections are invasive, relapsing or failing therapy, when inherent or acquired resistance is a possibility or when susceptibility cannot reliably be predicted from the species identification alone. Antifungal susceptibility testing (AFST) is also important in resistance surveillance, epidemiological studies and for comparison of the in vitro activity of new and existing agents. Dilution methods are used to establish the minimum inhibitory concentrations (MICs) of antimicrobial agents. They are the reference methods for antimicrobial susceptibility testing and are mainly used: to establish the activity of new antimicrobial agents, to confirm the susceptibility of organisms that give equivocal results in other test formats (such as commercial susceptibility tests), and to determine the susceptibility of organisms where other test formats may be unreliable or not yet validated (which is still a common scenario for susceptibility testing of moulds). In dilution tests, fungi are tested for their ability to produce sufficient growth in microdilution plate wells of broth culture media containing serial dilutions of the antimicrobial agents (broth microdilution). The antifungal MIC is defined as the lowest concentration, recorded in mg/L, of an agent that inhibits the growth of a fungus. The MIC informs about the susceptibility or resistance of the organism to the antifungal agent, which can help in treatment decisions. The increasing number of options for treating invasive mould disease, coupled with documented resistance to antifungal agents among some strains and species, has confirmed the need for having standardised methods for determining the in vitro susceptibilities of both new and established antifungal agents against clinical isolates of filamentous fungi [1-10]. The first version of the standard was published as the definitive document in July 2008 [11]. The second version was updated to reflect current knowledge regarding indications for mould AFST, stability of the echinocandins, inoculum preparation specifically for Aspergillus (spectrophotometer standardization of inoculum concentration was included), practical tips regarding endpoint reading (picture illustrations of endpoint reading), and interpretation of endpoints (acknowledging the established clinical breakpoints) [12]. This third version has been harmonised with the yeast E.Def 7.3.2 document, some technical details have been included, references have been updated to reflect contemporary knowledge, the revised definition of the I category has been introduced and MIC ranges for quality control strains have been removed. The latter has been done acknowledging the new separate document summarising all antifungal MIC ranges for quality control strains (available on the EUCAST website http://www.EUCAST.org) and in order to avoid a need for method document updating whenever a new QC range is established. Furthermore, the section concerning

  • Název v anglickém jazyce

    Method for the determination of broth dilution minimum inhibitory concetrations of antifungal agents for conidia forming moulds

  • Popis výsledku anglicky

    Antifungal susceptibility tests are performed on fungi causing disease especially when infections are invasive, relapsing or failing therapy, when inherent or acquired resistance is a possibility or when susceptibility cannot reliably be predicted from the species identification alone. Antifungal susceptibility testing (AFST) is also important in resistance surveillance, epidemiological studies and for comparison of the in vitro activity of new and existing agents. Dilution methods are used to establish the minimum inhibitory concentrations (MICs) of antimicrobial agents. They are the reference methods for antimicrobial susceptibility testing and are mainly used: to establish the activity of new antimicrobial agents, to confirm the susceptibility of organisms that give equivocal results in other test formats (such as commercial susceptibility tests), and to determine the susceptibility of organisms where other test formats may be unreliable or not yet validated (which is still a common scenario for susceptibility testing of moulds). In dilution tests, fungi are tested for their ability to produce sufficient growth in microdilution plate wells of broth culture media containing serial dilutions of the antimicrobial agents (broth microdilution). The antifungal MIC is defined as the lowest concentration, recorded in mg/L, of an agent that inhibits the growth of a fungus. The MIC informs about the susceptibility or resistance of the organism to the antifungal agent, which can help in treatment decisions. The increasing number of options for treating invasive mould disease, coupled with documented resistance to antifungal agents among some strains and species, has confirmed the need for having standardised methods for determining the in vitro susceptibilities of both new and established antifungal agents against clinical isolates of filamentous fungi [1-10]. The first version of the standard was published as the definitive document in July 2008 [11]. The second version was updated to reflect current knowledge regarding indications for mould AFST, stability of the echinocandins, inoculum preparation specifically for Aspergillus (spectrophotometer standardization of inoculum concentration was included), practical tips regarding endpoint reading (picture illustrations of endpoint reading), and interpretation of endpoints (acknowledging the established clinical breakpoints) [12]. This third version has been harmonised with the yeast E.Def 7.3.2 document, some technical details have been included, references have been updated to reflect contemporary knowledge, the revised definition of the I category has been introduced and MIC ranges for quality control strains have been removed. The latter has been done acknowledging the new separate document summarising all antifungal MIC ranges for quality control strains (available on the EUCAST website http://www.EUCAST.org) and in order to avoid a need for method document updating whenever a new QC range is established. Furthermore, the section concerning

Klasifikace

  • Druh

    N<sub>metS</sub> - Metodiky schválené orgánem státní správy

  • CEP obor

  • OECD FORD obor

    10606 - Microbiology

Návaznosti výsledku

  • Projekt

  • Návaznosti

    V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Ostatní

  • Rok uplatnění

    2020

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Interní identifikační kód produktu

    Vláknité houby

  • Číslo předpisu

    E.DEF 9.3.2

  • Technické parametry

    E.DEF 9.3.2, EUCAST AFST

  • Ekonomické parametry

    nová verze Evropského konsenzu testování citlivosti k ATB

  • Označení certifikačního orgánu

    Eucast

  • Datum certifikace

  • Způsoby využití výsledku

    C - Výsledek je využíván bez omezení okruhu uživatelů