When activator and inhibitor of PPARα do the same: Consequence for differentiation of human intestinal cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F21%3A73611451" target="_blank" >RIV/61989592:15110/21:73611451 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2227-9059/9/9/1255/htm" target="_blank" >https://www.mdpi.com/2227-9059/9/9/1255/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/biomedicines9091255" target="_blank" >10.3390/biomedicines9091255</a>

Jazyk výsledku

angličtina

Název v původním jazyce

When activator and inhibitor of PPARα do the same: Consequence for differentiation of human intestinal cells

Popis výsledku v původním jazyce

Peroxisome proliferator-activated receptor α (PPARα) is a ligand-dependent transcription factor which play a role in various processes, including differentiation of several cell types. We investigated the role of PPARα in differentiation of intestinal cells using HT-29 cell line as a model as well as human normal colon and colorectal carcinoma tissues. We detected significant increase in PPARα expression in differentiated HT-29 cells as well as in normal surface colon epithelium where differentiated cells are localized. Thus, it seemed that PPARα may play a role in differentiation of intestinal cells. Interestingly, we found that both, PPARα activator fenofibrate as well as its inhibitor GW6471 regulated proliferation and differentiation of HT-29 cells in vitro in the same way. Both compounds led to decrease in proliferation accompanied by significant increase in expression of villin, intestinal alkaline phosphatase (differentiation markers) together with increase in lipid droplets accumulation. Furthermore, villin expression was independent on subcellular localization of PPARα. Moreover, we found similar levels of PPARα expression in colorectal carcinomas in comparison to adjacent normal epithelium. All these finding support the hypothesis, that differentiation of intestinal epithelium is PPARα-independent.

Název v anglickém jazyce

When activator and inhibitor of PPARα do the same: Consequence for differentiation of human intestinal cells

Popis výsledku anglicky

Peroxisome proliferator-activated receptor α (PPARα) is a ligand-dependent transcription factor which play a role in various processes, including differentiation of several cell types. We investigated the role of PPARα in differentiation of intestinal cells using HT-29 cell line as a model as well as human normal colon and colorectal carcinoma tissues. We detected significant increase in PPARα expression in differentiated HT-29 cells as well as in normal surface colon epithelium where differentiated cells are localized. Thus, it seemed that PPARα may play a role in differentiation of intestinal cells. Interestingly, we found that both, PPARα activator fenofibrate as well as its inhibitor GW6471 regulated proliferation and differentiation of HT-29 cells in vitro in the same way. Both compounds led to decrease in proliferation accompanied by significant increase in expression of villin, intestinal alkaline phosphatase (differentiation markers) together with increase in lipid droplets accumulation. Furthermore, villin expression was independent on subcellular localization of PPARα. Moreover, we found similar levels of PPARα expression in colorectal carcinomas in comparison to adjacent normal epithelium. All these finding support the hypothesis, that differentiation of intestinal epithelium is PPARα-independent.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedicines

ISSN

2227-9059

e-ISSN

—

Svazek periodika

2021

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

17

Strana od-do

"nestránkováno"

Kód UT WoS článku

000699234400001

EID výsledku v databázi Scopus

2-s2.0-85115798949