Soluble epoxide hydrolase as an important player in intestinal cell differentiation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F21%3A73611535" target="_blank" >RIV/61989592:15110/21:73611535 - isvavai.cz</a>

Výsledek na webu

<a href="https://obd.upol.cz/id_publ/333191422" target="_blank" >https://obd.upol.cz/id_publ/333191422</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1159/000512807" target="_blank" >10.1159/000512807</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Soluble epoxide hydrolase as an important player in intestinal cell differentiation

Popis výsledku v původním jazyce

There is a growing evidence that soluble epoxide hydrolase (sEH) may play a role in cell differentiation. sEH metabolizes biologically highly active and generally cytoprotective epoxyeicosatrienoic acids (EETs), generated from arachidonic acid metabolism by CYP epoxygenases (CYP2C and CYP2J subfamilies), to less active corresponding diols. We investigated the effect of sEH inhibitor (TPPU) on expression of villin, CYP2C8, CYP2C9, CYP2J2 and sEH in undifferentiated and in vitro differentiated HT-29 and Caco2 cell lines. The administration of 10 μM TPPU on differentiated HT-29 and Caco2 cells resulted in a significant decrease in expression of villin, marker of intestinal cell differentiation. It was accompanied by disruption of brush border when microvilli appeared sparse and short in atomic force microscope scans of HT-29 cells. Although inhibition of sEH in differentiated HT-29 and Caco2 cells led to increase in sEH expression in both cell lines, this treatment had an opposite effect on CYP2J2 expression in HT-29 and Caco2. In addition, tissue samples of colorectal carcinoma and adjacent normal tissues from 45 patients were immunostained for sEH and villin. We detected a significant decrease in expression of both proteins in colorectal carcinoma in comparison to adjacent normal tissue, and the decrease in both sEH and villin expression revealed moderate positive association. Taken together, our results proved that sEH is an important player in intestinal cell differentiation.

Název v anglickém jazyce

Soluble epoxide hydrolase as an important player in intestinal cell differentiation

Popis výsledku anglicky

There is a growing evidence that soluble epoxide hydrolase (sEH) may play a role in cell differentiation. sEH metabolizes biologically highly active and generally cytoprotective epoxyeicosatrienoic acids (EETs), generated from arachidonic acid metabolism by CYP epoxygenases (CYP2C and CYP2J subfamilies), to less active corresponding diols. We investigated the effect of sEH inhibitor (TPPU) on expression of villin, CYP2C8, CYP2C9, CYP2J2 and sEH in undifferentiated and in vitro differentiated HT-29 and Caco2 cell lines. The administration of 10 μM TPPU on differentiated HT-29 and Caco2 cells resulted in a significant decrease in expression of villin, marker of intestinal cell differentiation. It was accompanied by disruption of brush border when microvilli appeared sparse and short in atomic force microscope scans of HT-29 cells. Although inhibition of sEH in differentiated HT-29 and Caco2 cells led to increase in sEH expression in both cell lines, this treatment had an opposite effect on CYP2J2 expression in HT-29 and Caco2. In addition, tissue samples of colorectal carcinoma and adjacent normal tissues from 45 patients were immunostained for sEH and villin. We detected a significant decrease in expression of both proteins in colorectal carcinoma in comparison to adjacent normal tissue, and the decrease in both sEH and villin expression revealed moderate positive association. Taken together, our results proved that sEH is an important player in intestinal cell differentiation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

CELLS TISSUES ORGANS

ISSN

1422-6405

e-ISSN

—

Svazek periodika

209

Číslo periodika v rámci svazku

4-6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

177-188

Kód UT WoS článku

000619097700001

EID výsledku v databázi Scopus

2-s2.0-85100902457