Replacement of DAPI with propidium iodide could extend the utilisation of archival tissue samples for immunofluorescent techniques

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F22%3A73617619" target="_blank" >RIV/61989592:15110/22:73617619 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1111/his.14676" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/his.14676</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/his.14676" target="_blank" >10.1111/his.14676</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Replacement of DAPI with propidium iodide could extend the utilisation of archival tissue samples for immunofluorescent techniques

Popis výsledku v původním jazyce

Archival tissues are rich source of potential material for retrospective and comparative studies, which may lead to identification of novel biomarkers with prognostic and/or predictive character. Using immunofluorescence, we experienced blur, low quality of DAPI staining of nuclei in several archival samples. Because the clear recognition of nuclei is crucial in immunofluorescent techniques, we systematically investigated the effect of tissue block aging on quality of nuclear staining. In archival placental tissue samples stored for 1, 5, 18, and 26 years, DAPI staining was examined. Although DAPI is widely used in immunofluorescent staining, we tried to replaced it by propidium iodide. ImageJ software was used for recognition of nuclei. Moreover, to confirm of tissue integrity, the same samples were stained with haematoxylin. Our results showed that although the haematoxylin staining showed distinct quality in all samples, DAPI straining deteriorated with increasing age of tissue blocks and this phenomenon could be improved using propidium iodide. Replacement of DAPI by propidium iodide significantly increased detectability of cell nuclei in tissue blocks stored for 18 or 26 years by ImageJ software. Based on our results, we suppose that substitution of DAPI by propidium iodide could extend the utilization of immunofluorescent techniques to archival tissue samples.

Název v anglickém jazyce

Replacement of DAPI with propidium iodide could extend the utilisation of archival tissue samples for immunofluorescent techniques

Popis výsledku anglicky

Archival tissues are rich source of potential material for retrospective and comparative studies, which may lead to identification of novel biomarkers with prognostic and/or predictive character. Using immunofluorescence, we experienced blur, low quality of DAPI staining of nuclei in several archival samples. Because the clear recognition of nuclei is crucial in immunofluorescent techniques, we systematically investigated the effect of tissue block aging on quality of nuclear staining. In archival placental tissue samples stored for 1, 5, 18, and 26 years, DAPI staining was examined. Although DAPI is widely used in immunofluorescent staining, we tried to replaced it by propidium iodide. ImageJ software was used for recognition of nuclei. Moreover, to confirm of tissue integrity, the same samples were stained with haematoxylin. Our results showed that although the haematoxylin staining showed distinct quality in all samples, DAPI straining deteriorated with increasing age of tissue blocks and this phenomenon could be improved using propidium iodide. Replacement of DAPI by propidium iodide significantly increased detectability of cell nuclei in tissue blocks stored for 18 or 26 years by ImageJ software. Based on our results, we suppose that substitution of DAPI by propidium iodide could extend the utilization of immunofluorescent techniques to archival tissue samples.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

HISTOPATHOLOGY

ISSN

0309-0167

e-ISSN

1365-2559

Svazek periodika

81

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

3

Strana od-do

409-411

Kód UT WoS článku

000797391000001

EID výsledku v databázi Scopus

2-s2.0-85129901171