2-D gel densitometer for high-contrast and selective imaging of chlorophyll-containing protein complexes separated by non-denaturing polyacrylamide gel electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F02%3A00001521" target="_blank" >RIV/61989592:15310/02:00001521 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

2-D gel densitometer for high-contrast and selective imaging of chlorophyll-containing protein complexes separated by non-denaturing polyacrylamide gel electrophoresis

Popis výsledku v původním jazyce

In this work we present a home-made two-dimensional CCD imaging system for the monochromatic densitometry of plane gels and its application to the imaging and densitometry of chlorophyll-containing proteins separated by non-denaturing PAGE. The monochromatic imaging of separated green bands at the wavelengths corresponding to their absorption band increases their contrast. This allows a better visualization of the faint-green bands in the gel and using of samples with lower chlorophyll (Chl) content forthe electrophoresis. By the comparison of 2-D densitograms of the same gel illuminated with 670 and 650 nm lights, i.e. at the red absorption maximum of Chl a and b, respectively, we achieved a selective imaging of the complexes with different Chl a/b ratio. This approach was used to specify an unknown band that appeared in the gel of the sample prepared from the thylakoid membranes of preheated barley leaves.

Název v anglickém jazyce

2-D gel densitometer for high-contrast and selective imaging of chlorophyll-containing protein complexes separated by non-denaturing polyacrylamide gel electrophoresis

Popis výsledku anglicky

In this work we present a home-made two-dimensional CCD imaging system for the monochromatic densitometry of plane gels and its application to the imaging and densitometry of chlorophyll-containing proteins separated by non-denaturing PAGE. The monochromatic imaging of separated green bands at the wavelengths corresponding to their absorption band increases their contrast. This allows a better visualization of the faint-green bands in the gel and using of samples with lower chlorophyll (Chl) content forthe electrophoresis. By the comparison of 2-D densitograms of the same gel illuminated with 670 and 650 nm lights, i.e. at the red absorption maximum of Chl a and b, respectively, we achieved a selective imaging of the complexes with different Chl a/b ratio. This approach was used to specify an unknown band that appeared in the gel of the sample prepared from the thylakoid membranes of preheated barley leaves.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biochemical and Biophysical Methods

ISSN

0165-022X

e-ISSN

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Svazek periodika

51

Číslo periodika v rámci svazku

N

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

273-281

Kód UT WoS článku

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EID výsledku v databázi Scopus

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