Cytokinin biosynthesis and Interaction with Auxin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F03%3A00001585" target="_blank" >RIV/61989592:15310/03:00001585 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cytokinin biosynthesis and Interaction with Auxin

Popis výsledku v původním jazyce

Auxin-cytokinin intewractions were investigated in Arabidopsis thaliana and Nicotiana tabacum by the use of in-vivo deuterium labeling and mass spectrometry. 36 hours after incubation of cytokinin overproduction in transgenic Arabidopsis thaliana plants,significant lower endogenous IAA biosynthetic rates and pool sizes were detected. In the next set of experiments, the auxin NAA was shown to reduce cytokinin biosynthetic rate in Arabidopsis wt plants within 6h of incubation. In contrast to the cytokinin-auxin case, this rapid effect indicates a direct on transcriptional or translation of synthase related genes. Furthermore, NAA was found exclusively act on the alternative, IPMP-independent pathway of cytokinin biosynthesis producing ZMP not involvingiP-type of cytokinins as intermediates. The de novo biosynthetic activity in shoot tissues was studied in tobacco leaves of different developmental stages. The highest rates of de novo ZMP biosynthesis were found in very young leaves sugge

Název v anglickém jazyce

Cytokinin biosynthesis and Interaction with Auxin

Popis výsledku anglicky

Auxin-cytokinin intewractions were investigated in Arabidopsis thaliana and Nicotiana tabacum by the use of in-vivo deuterium labeling and mass spectrometry. 36 hours after incubation of cytokinin overproduction in transgenic Arabidopsis thaliana plants,significant lower endogenous IAA biosynthetic rates and pool sizes were detected. In the next set of experiments, the auxin NAA was shown to reduce cytokinin biosynthetic rate in Arabidopsis wt plants within 6h of incubation. In contrast to the cytokinin-auxin case, this rapid effect indicates a direct on transcriptional or translation of synthase related genes. Furthermore, NAA was found exclusively act on the alternative, IPMP-independent pathway of cytokinin biosynthesis producing ZMP not involvingiP-type of cytokinins as intermediates. The de novo biosynthetic activity in shoot tissues was studied in tobacco leaves of different developmental stages. The highest rates of de novo ZMP biosynthesis were found in very young leaves sugge

Druh

D - Stať ve sborníku

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F01%2F0275" target="_blank" >GA522/01/0275: Studium nových cytokininových metabolitů v rostlinách: isolace, identifikace a biosyntéza</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Plant Biology 2003 - the 2003 annual meeting of the American Society of Plant Biology

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

286

Strana od-do

141

Název nakladatele

American Society of Plant Physiologists

Místo vydání

Honolulu

Místo konání akce

Honolulu

Datum konání akce

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Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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