Charakterizace cytokinindehydrogenasy z Arabidopsis thaliana exprimované v kvasince Saccharomyces cerevisiae a rostlinách tabáku

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002103" target="_blank" >RIV/61989592:15310/04:00002103 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of Arabidopsis cytokinin dehydrogenase expressed in yeast Saccharomyces cerevisiae and tobacco plants

Popis výsledku v původním jazyce

Cytokinin dehydrogenase (CKX, EC 1.5.99.12) controls cytokinin levels in plants by oxidative cleavage of their side chain. The amount of cytokinin dehydrogenase in plants is generally very low and its isolation and purification is thus challenging. The cloning of CKX gene from maize1,2 opened a new opportunity for study of CKX and cytokinin function in plants. In Arabidopsis thaliana, seven distinct genes coding for seven proteins (AtCKX1 to AtCKX7) were found. In present study we aim to prepare yeast strains for production of recombinant AtCKX2 and to characterize CKX enzymes in tobacco plants overexpressing individual enzymes from Arabidopsis thaliana. AtCKX2 gene was cloned into pYES2 and pDR197 yeast expression plasmids or integrated into yeast genome using vectors YIplac211 and pYESX. The Saccharomyces cerevisiae strain 23344c (MAT#Á ura3) was used. CKX was secreted into the media in all tested yeast systems, the constitutive and inducible promoter driven expression showed compara

Název v anglickém jazyce

Characterization of Arabidopsis cytokinin dehydrogenase expressed in yeast Saccharomyces cerevisiae and tobacco plants

Popis výsledku anglicky

Cytokinin dehydrogenase (CKX, EC 1.5.99.12) controls cytokinin levels in plants by oxidative cleavage of their side chain. The amount of cytokinin dehydrogenase in plants is generally very low and its isolation and purification is thus challenging. The cloning of CKX gene from maize1,2 opened a new opportunity for study of CKX and cytokinin function in plants. In Arabidopsis thaliana, seven distinct genes coding for seven proteins (AtCKX1 to AtCKX7) were found. In present study we aim to prepare yeast strains for production of recombinant AtCKX2 and to characterize CKX enzymes in tobacco plants overexpressing individual enzymes from Arabidopsis thaliana. AtCKX2 gene was cloned into pYES2 and pDR197 yeast expression plasmids or integrated into yeast genome using vectors YIplac211 and pYESX. The Saccharomyces cerevisiae strain 23344c (MAT#Á ura3) was used. CKX was secreted into the media in all tested yeast systems, the constitutive and inducible promoter driven expression showed compara

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F03%2F0979" target="_blank" >GA522/03/0979: Organizace genů cytokinin dehydrogenázy v obilovinách a mechanismus katalytické reakce odbourávání cytokininů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Universitatis Palackianae Olomucensis, Facultas Rerum Naturalium, Chemica

ISSN

0232-0061

e-ISSN

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Svazek periodika

43

Číslo periodika v rámci svazku

S

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

2

Strana od-do

38-39

Kód UT WoS článku

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EID výsledku v databázi Scopus

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