Exprese cytokinindehydrogenasy z Arabidopsis thaliana v kvasince Saccharomyces cerevisiae a tabáku

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002104" target="_blank" >RIV/61989592:15310/04:00002104 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Expression of Arabidopsis thaliana cytokinin dehydrogenase in yeast Saccharomyces cerevisiae and tobacco plants

Popis výsledku v původním jazyce

Introduction: Cytokinin dehydrogenase (CKX, EC 1.5.99.12) controls cytokinin levels in plants by oxidative cleavage of their side chain. The amount of cytokinin dehydrogenase in plants is generally very low and its isolation and purification is thus challenging. The cloning of CKX gene from maize opened a new opportunity for study of CKX and cytokinin function in plants. The aim of this study was to prepare yeast strains for production of recombinant CKX and to characterize CKX enzymes in tobacco plantsoverexpressing AtCKXs.Material and Methods: AtCKX2 gene was cloned into pYES2 and pDR197 yeast expression plasmids or integrated into yeast genome using vectors YIplac211 and pYESX. The Saccharomyces cerevisiae strain 23344c (MAT&#61537; ura3) was used.CKX activity was assayed according to Frébort etal., (2002). Leaf extracts of wild-type and transgenic Nicotiana tabaccum L. cv. Samsun NN overexpressing Arabidopsis CKX genes 1 to 4, (Werner etal., 2001), 6 and 7, were used for characte

Název v anglickém jazyce

Expression of Arabidopsis thaliana cytokinin dehydrogenase in yeast Saccharomyces cerevisiae and tobacco plants

Popis výsledku anglicky

Introduction: Cytokinin dehydrogenase (CKX, EC 1.5.99.12) controls cytokinin levels in plants by oxidative cleavage of their side chain. The amount of cytokinin dehydrogenase in plants is generally very low and its isolation and purification is thus challenging. The cloning of CKX gene from maize opened a new opportunity for study of CKX and cytokinin function in plants. The aim of this study was to prepare yeast strains for production of recombinant CKX and to characterize CKX enzymes in tobacco plantsoverexpressing AtCKXs.Material and Methods: AtCKX2 gene was cloned into pYES2 and pDR197 yeast expression plasmids or integrated into yeast genome using vectors YIplac211 and pYESX. The Saccharomyces cerevisiae strain 23344c (MAT&#61537; ura3) was used.CKX activity was assayed according to Frébort etal., (2002). Leaf extracts of wild-type and transgenic Nicotiana tabaccum L. cv. Samsun NN overexpressing Arabidopsis CKX genes 1 to 4, (Werner etal., 2001), 6 and 7, were used for characte

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F03%2F0979" target="_blank" >GA522/03/0979: Organizace genů cytokinin dehydrogenázy v obilovinách a mechanismus katalytické reakce odbourávání cytokininů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Physiologiae Plantarum

ISSN

0137-5881

e-ISSN

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Svazek periodika

26

Číslo periodika v rámci svazku

S

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

1

Strana od-do

37

Kód UT WoS článku

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EID výsledku v databázi Scopus

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