Použití Q-TOF technologie při studiu rostlinných hormonů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002217" target="_blank" >RIV/61989592:15310/04:00002217 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Application of Q-TOF technology in plant hormone research

Popis výsledku v původním jazyce

Combination of high performance liquid chromatography (HPLC) or capillary liquid chromatography (CapLC) with quadrupole-time of flight mass spectrometry (Q-TOF) makes possible a new direction in plant hormone research. The instrument facilities are a good alternative for search of new plant metabolites and metabolic pathways. The recently developed method for quantitative analysis of natural cytokinins (Novák et al., 2003) was adapted from single-quadrupole mass spectrometer (SQ-MS) to Q-TOF micro instrument. The full scan spectra at very low concentration levels (100 fmol) were obtained together with MS-MS analyses of selected ions. The accurate masses of the parent ions and their fragments were calculated and used for determination of elementary structure with fidelity 10 ppm and better with a external calibration. The optimal conditions for combination of HPLC and CapLC with Q-TOF, i.e. capillary and cone voltage, desolvation gas flow and temperature for effective ionization and col

Název v anglickém jazyce

Application of Q-TOF technology in plant hormone research

Popis výsledku anglicky

Combination of high performance liquid chromatography (HPLC) or capillary liquid chromatography (CapLC) with quadrupole-time of flight mass spectrometry (Q-TOF) makes possible a new direction in plant hormone research. The instrument facilities are a good alternative for search of new plant metabolites and metabolic pathways. The recently developed method for quantitative analysis of natural cytokinins (Novák et al., 2003) was adapted from single-quadrupole mass spectrometer (SQ-MS) to Q-TOF micro instrument. The full scan spectra at very low concentration levels (100 fmol) were obtained together with MS-MS analyses of selected ions. The accurate masses of the parent ions and their fragments were calculated and used for determination of elementary structure with fidelity 10 ppm and better with a external calibration. The optimal conditions for combination of HPLC and CapLC with Q-TOF, i.e. capillary and cone voltage, desolvation gas flow and temperature for effective ionization and col

Druh

D - Stať ve sborníku

CEP obor

EF - Botanika

OECD FORD obor

—

Projekt

<a href="/cs/project/GA522%2F03%2F0323" target="_blank" >GA522/03/0323: Studium produkce biologicky aktivních látek řasami a sinicemi</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

18th IPGSA conference, Conference Handbook

ISBN

—

ISSN

—

e-ISSN

—

Počet stran výsledku

144

Strana od-do

124

Název nakladatele

Australian National University

Místo vydání

Canberra

Místo konání akce

—

Datum konání akce

—

Typ akce podle státní příslušnosti

—

Kód UT WoS článku

—