Kvantitavní stanovení cytokininů v rostlinách metodou kapalinové chromatografie-hmotnostní spektrometrie

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002219" target="_blank" >RIV/61989592:15310/04:00002219 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Quantitative analysis of cytokinins in plants by liquid chromatography/single-quadrupole mass spectrometry

Popis výsledku v původním jazyce

The method for estimation of 37 naturally occurring cytokinins is based on an immunoaffinity purification (IAC) and a baseline chromatographic separation of 20 cytokinins (trans/cis-zeatin, dihydrozeatin, isopentenyladenine, benzyladenine, meta/ortho-topoline groups) on reversed-phase column and analysis by high-performance liquid chromatography-mass spectrometry (HPLC/MS). When low cone voltage (20 V) was applied, all studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of [M+H]+ with limits of detection between 10 and 50 fmol and with linearity ranges of calibration between 25 fmol and 100 pmol. The method was validated by deuterium or 15N labeled standards (isoprenoid and aromatic), added during extraction. Two different extraction procedures (70% ethanol or Bieleski buffer) have been tested. Bieleski extracts were purified by two different ion-exchange chromatography steps (SCX and DEAE Sephadex) in combination with immunoaffinity purification bas

Název v anglickém jazyce

Quantitative analysis of cytokinins in plants by liquid chromatography/single-quadrupole mass spectrometry

Popis výsledku anglicky

The method for estimation of 37 naturally occurring cytokinins is based on an immunoaffinity purification (IAC) and a baseline chromatographic separation of 20 cytokinins (trans/cis-zeatin, dihydrozeatin, isopentenyladenine, benzyladenine, meta/ortho-topoline groups) on reversed-phase column and analysis by high-performance liquid chromatography-mass spectrometry (HPLC/MS). When low cone voltage (20 V) was applied, all studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of [M+H]+ with limits of detection between 10 and 50 fmol and with linearity ranges of calibration between 25 fmol and 100 pmol. The method was validated by deuterium or 15N labeled standards (isoprenoid and aromatic), added during extraction. Two different extraction procedures (70% ethanol or Bieleski buffer) have been tested. Bieleski extracts were purified by two different ion-exchange chromatography steps (SCX and DEAE Sephadex) in combination with immunoaffinity purification bas

Druh

D - Stať ve sborníku

CEP obor

EF - Botanika

OECD FORD obor

—

Projekt

<a href="/cs/project/GA522%2F03%2F0323" target="_blank" >GA522/03/0323: Studium produkce biologicky aktivních látek řasami a sinicemi</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

18th IPGSA conference, Conference Handbook

ISBN

—

ISSN

—

e-ISSN

—

Počet stran výsledku

144

Strana od-do

124

Název nakladatele

Australian National University

Místo vydání

Canberra

Místo konání akce

—

Datum konání akce

—

Typ akce podle státní příslušnosti

—

Kód UT WoS článku

—