Nové metohologické přístupy v analýzách rostlinných hormonů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002446" target="_blank" >RIV/61989592:15310/04:00002446 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

New methodological approaches to plant hormone analysis

Popis výsledku v původním jazyce

New approach in the analytical technologies of plant hormone analysis was introduced in the field of cytokinins and might be also soon applied in the analysis of other plant hormone groups. A new sensitive method for the quantitative analysis of all natural isoprenoid and aromatic cytokinins in plants is based on combined "generic" immunoaffinity chromatography - electrospray single-quadrupole mass. A baseline chromatographic separation of 20 non-derivatized naturally occurring cytokinins has been obtained. Precise analysis of O-glucoside and nucleotide fractions were also performed by the HPLC-MS runs but after an enzymatic treatment. More than 50 different cytokinin metabolites can be measured by this approach. When low cone voltage (20 eV) was applied, all studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of M+H + with limits of detection from 10 to 50 fmol. For routine analysis we obtained a linearity range between 25 (75) fmol - 100 pmol. Deve

Název v anglickém jazyce

New methodological approaches to plant hormone analysis

Popis výsledku anglicky

New approach in the analytical technologies of plant hormone analysis was introduced in the field of cytokinins and might be also soon applied in the analysis of other plant hormone groups. A new sensitive method for the quantitative analysis of all natural isoprenoid and aromatic cytokinins in plants is based on combined "generic" immunoaffinity chromatography - electrospray single-quadrupole mass. A baseline chromatographic separation of 20 non-derivatized naturally occurring cytokinins has been obtained. Precise analysis of O-glucoside and nucleotide fractions were also performed by the HPLC-MS runs but after an enzymatic treatment. More than 50 different cytokinin metabolites can be measured by this approach. When low cone voltage (20 eV) was applied, all studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of M+H + with limits of detection from 10 to 50 fmol. For routine analysis we obtained a linearity range between 25 (75) fmol - 100 pmol. Deve

Druh

D - Stať ve sborníku

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F01%2F0275" target="_blank" >GA522/01/0275: Studium nových cytokininových metabolitů v rostlinách: isolace, identifikace a biosyntéza</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

18th IPGSA conference, Conference Handbook

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

144

Strana od-do

87

Název nakladatele

Australian National University

Místo vydání

Canberra

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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