Cytokininy a fenolové látky v rostlinách: kooperativní metabolismus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00002621" target="_blank" >RIV/61989592:15310/06:00002621 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cytokinins and Plant Phenolics: A Cooperative Metabolism

Popis výsledku v původním jazyce

Cytokinin dehydrogenase (CKX) is a key enzyme in regulation of the levels of active cytokinins in plants. We have previously reported that CKX can use products of oxidation of plant phenolics by tyrosinase and peroxidase as acceptors of electrons from oxidized cytokinin and that the phloem sap of maize seedlings contains unknown phenolic precursor of this acceptor. In the present work we investigated a set of substituted phenolic acids for their ability to serve as precursors of electron acceptors in the CKX reaction and analyzed the phloem sap of maize seedlings for the presence of carboxylic phenolic acids using HPLC with a Q-TOF detector. Only p-coumaric, caffeic and chlorogenic acids were converted to effective electron acceptors in the presence ofmushroom tyrosinase. Ferulic acid showed weak activity and analogous compounds derived from 4-hydroxybenzoic acid were not active. Compounds effective in in vitro CKX assay were identified in the phloem sap.

Název v anglickém jazyce

Cytokinins and Plant Phenolics: A Cooperative Metabolism

Popis výsledku anglicky

Cytokinin dehydrogenase (CKX) is a key enzyme in regulation of the levels of active cytokinins in plants. We have previously reported that CKX can use products of oxidation of plant phenolics by tyrosinase and peroxidase as acceptors of electrons from oxidized cytokinin and that the phloem sap of maize seedlings contains unknown phenolic precursor of this acceptor. In the present work we investigated a set of substituted phenolic acids for their ability to serve as precursors of electron acceptors in the CKX reaction and analyzed the phloem sap of maize seedlings for the presence of carboxylic phenolic acids using HPLC with a Q-TOF detector. Only p-coumaric, caffeic and chlorogenic acids were converted to effective electron acceptors in the presence ofmushroom tyrosinase. Ferulic acid showed weak activity and analogous compounds derived from 4-hydroxybenzoic acid were not active. Compounds effective in in vitro CKX assay were identified in the phloem sap.

Druh

C - Kapitola v odborné knize

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F05%2F0448" target="_blank" >GA522/05/0448: Izolace a identifikace přirozeného elektronového akceptoru rostlinné cytokinindehydrogenasy a její interakce s nízkomolekulárními látkami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues

ISBN

978-4-903313-00-9

Počet stran výsledku

646

Strana od-do

379-384

Počet stran knihy

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Název nakladatele

Global Science Books

Místo vydání

Isleworth

Kód UT WoS kapitoly

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