Reakční cesty produkce hydroxylových radikálů v thylakoidní membráně: spin-trapping EPR studie.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00003237" target="_blank" >RIV/61989592:15310/06:00003237 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Reaction pathways involved in the production of hydroxyl radicals in thylakoid membrane: EPR spin-trapping study.

Popis výsledku v původním jazyce

It has been suggested that both free metals and reduced ferredoxin (Fd) participate in the light-induced production of hydroxyl radicals (OH.) in thylakoid membranes of chloroplasts. The most direct evidence of the involvement of Fd in OH. formation under physiological conditions was reported by Jakob and Heber (Plant Cell Physiol., 1996, 37, 629-635), who used the oxidation of dimethylsulfoxide to methane sulfinic acid as an indicator of OH. production. We confirmed their conclusions using a more sensitive and reliable EPR spin-trapping method and extended their work by additional findings. Free metal-dependent and ferredoxin-dependent OH. production was studied simultaneously and strong metal chelator Desferal was used to distinguish between these reaction pathways. The participation of protein-bound iron within photosystem I was confirmed by partial suppression of OH. generation in broken chloroplasts by methyl viologen. The stimulation of the production of OH. in thylakoid membrane

Název v anglickém jazyce

Reaction pathways involved in the production of hydroxyl radicals in thylakoid membrane: EPR spin-trapping study.

Popis výsledku anglicky

It has been suggested that both free metals and reduced ferredoxin (Fd) participate in the light-induced production of hydroxyl radicals (OH.) in thylakoid membranes of chloroplasts. The most direct evidence of the involvement of Fd in OH. formation under physiological conditions was reported by Jakob and Heber (Plant Cell Physiol., 1996, 37, 629-635), who used the oxidation of dimethylsulfoxide to methane sulfinic acid as an indicator of OH. production. We confirmed their conclusions using a more sensitive and reliable EPR spin-trapping method and extended their work by additional findings. Free metal-dependent and ferredoxin-dependent OH. production was studied simultaneously and strong metal chelator Desferal was used to distinguish between these reaction pathways. The participation of protein-bound iron within photosystem I was confirmed by partial suppression of OH. generation in broken chloroplasts by methyl viologen. The stimulation of the production of OH. in thylakoid membrane

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/1K04103" target="_blank" >1K04103: Reaktivní formy kyslíku ve fotosyntéze</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Photochemical and Photobiological Sciences

ISSN

1474-905X

e-ISSN

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Svazek periodika

5

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

472-476

Kód UT WoS článku

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EID výsledku v databázi Scopus

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