Hydroxylové radikály nejsou protagonisté poškození izolovaných thylakoidních membrán pomocí UV-B.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F07%3A00004841" target="_blank" >RIV/61989592:15310/07:00004841 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Hydroxyl radicals are not the protagonists of UV-B induced damage in isolated thylakoid membranes.

Popis výsledku v původním jazyce

The production of reactive oxygen species (ROS) was studied in isolated thylakoid membranes exposed to 312 nm UV-B irradiation. Hydroxyl radicals (-OH) and hydrogen peroxide were measured directly, using a newly developed method based on hydroxylation ofterephthalic acid and the homovanillic acid/peroxidase assay, respectively. At the early stage of UV-B stress (doses lower than 2.0 J cm/-2), -OH were derived from superoxide radicals via hydrogen peroxide. Production of these ROS was dependent on photosynthetic electron transport and was not exclusive to UV-B. Both ROS were found in samples exposed to the same doses of PAR, suggesting that the observed ROS are by-products of the UV-B driven electron transport rather than specific initiators of the UV-B-induced damage. After longer exposure of thylakoids to UV-B, leading to the inactivation of PSII centres, a small amount of -OH was still observed in thylakoids, even though no free hydrogen peroxide was detected.

Název v anglickém jazyce

Hydroxyl radicals are not the protagonists of UV-B induced damage in isolated thylakoid membranes.

Popis výsledku anglicky

The production of reactive oxygen species (ROS) was studied in isolated thylakoid membranes exposed to 312 nm UV-B irradiation. Hydroxyl radicals (-OH) and hydrogen peroxide were measured directly, using a newly developed method based on hydroxylation ofterephthalic acid and the homovanillic acid/peroxidase assay, respectively. At the early stage of UV-B stress (doses lower than 2.0 J cm/-2), -OH were derived from superoxide radicals via hydrogen peroxide. Production of these ROS was dependent on photosynthetic electron transport and was not exclusive to UV-B. Both ROS were found in samples exposed to the same doses of PAR, suggesting that the observed ROS are by-products of the UV-B driven electron transport rather than specific initiators of the UV-B-induced damage. After longer exposure of thylakoids to UV-B, leading to the inactivation of PSII centres, a small amount of -OH was still observed in thylakoids, even though no free hydrogen peroxide was detected.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Functional Plant Biology

ISSN

1445-4408

e-ISSN

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Svazek periodika

34

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

AU - Austrálie

Počet stran výsledku

10

Strana od-do

1112-1121

Kód UT WoS článku

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EID výsledku v databázi Scopus

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