Efekt kovových chelátorů na produkci hydroxylových radikálů v thylakoidech.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00003236" target="_blank" >RIV/61989592:15310/06:00003236 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of metal chelators on the production of hydroxyl radicals in thylakoids.

Popis výsledku v původním jazyce

The effect of metal chelators (EDTA, DTPA and Desferal) on the metal-catalyzed decomposition of hydrogen peroxide was studied using EPR spin-trapping spectroscopy. The formation of hydroxyl radicals (OH.) in both chemical (Fenton reaction) and biological(thylakoids) systems was stimulated by EDTA. DTPA promoted the generation of OH. in the presence of strong reducing agents, whereas in their absence it acted as an antioxidant. Desferal suppressed OH. production even in the presence of reductants. In our study we have shown that metal chelators can both stimulate and suppress the formation of OH., depending on the experimental conditions. In illuminated thylakoids we have observed prooxidant effect of EDTA and DTPA, possibly due to their reduction by some component of the electron transport chain. According to our results, metal chelators should not be used as antioxidants without prior testing of their effect in given samples.

Název v anglickém jazyce

The effect of metal chelators on the production of hydroxyl radicals in thylakoids.

Popis výsledku anglicky

The effect of metal chelators (EDTA, DTPA and Desferal) on the metal-catalyzed decomposition of hydrogen peroxide was studied using EPR spin-trapping spectroscopy. The formation of hydroxyl radicals (OH.) in both chemical (Fenton reaction) and biological(thylakoids) systems was stimulated by EDTA. DTPA promoted the generation of OH. in the presence of strong reducing agents, whereas in their absence it acted as an antioxidant. Desferal suppressed OH. production even in the presence of reductants. In our study we have shown that metal chelators can both stimulate and suppress the formation of OH., depending on the experimental conditions. In illuminated thylakoids we have observed prooxidant effect of EDTA and DTPA, possibly due to their reduction by some component of the electron transport chain. According to our results, metal chelators should not be used as antioxidants without prior testing of their effect in given samples.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/1K04103" target="_blank" >1K04103: Reaktivní formy kyslíku ve fotosyntéze</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Photosynthesis Research

ISSN

0166-8595

e-ISSN

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Svazek periodika

88

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

323-329

Kód UT WoS článku

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EID výsledku v databázi Scopus

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