Dark production of reactive oxygen species in photosystem II membrane particles at elevated temperature - EPR spin-trapping study.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F07%3A00004659" target="_blank" >RIV/61989592:15310/07:00004659 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Dark production of reactive oxygen species in photosystem II membrane particles at elevated temperature - EPR spin-trapping study.

Popis výsledku v původním jazyce

In our study, spin-trapping EPR technique was employed to study dark production of two reactive oxygen species, hydroxyl radicals (OH?) and singlet oxygen (1O2), in spinach PSII membrane particles exposed to elevated temperature (47 °C). Production of OH?, evaluated as EMPO-OH adduct EPR signal, was suppressed by the enzymatic removal of hydrogen peroxide and by the addition of iron chelator desferal, whereas externally added hydrogen peroxide enhanced OH? production. These observations reveal that OH?is presumably produced by metal-mediated reduction of hydrogen peroxide in a Fenton-type reaction. Increase in pH above physiological values significantly stimulated the formation of OH?, whereas the presence of chloride and calcium ions had the oppositeeffect. Based on our results it is proposed that the formation of OH? is linked to the thermal disassembly of water-splitting manganese complex on PSII donor side.

Název v anglickém jazyce

Dark production of reactive oxygen species in photosystem II membrane particles at elevated temperature - EPR spin-trapping study.

Popis výsledku anglicky

In our study, spin-trapping EPR technique was employed to study dark production of two reactive oxygen species, hydroxyl radicals (OH?) and singlet oxygen (1O2), in spinach PSII membrane particles exposed to elevated temperature (47 °C). Production of OH?, evaluated as EMPO-OH adduct EPR signal, was suppressed by the enzymatic removal of hydrogen peroxide and by the addition of iron chelator desferal, whereas externally added hydrogen peroxide enhanced OH? production. These observations reveal that OH?is presumably produced by metal-mediated reduction of hydrogen peroxide in a Fenton-type reaction. Increase in pH above physiological values significantly stimulated the formation of OH?, whereas the presence of chloride and calcium ions had the oppositeeffect. Based on our results it is proposed that the formation of OH? is linked to the thermal disassembly of water-splitting manganese complex on PSII donor side.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/1K04103" target="_blank" >1K04103: Reaktivní formy kyslíku ve fotosyntéze</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica Et Biophysica Acta - Bioenergetics

ISSN

0005-2728

e-ISSN

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Svazek periodika

1767

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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