Tvorba reaktivních forem kyslíku fotosystémem II.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F05%3A00002229" target="_blank" >RIV/61989592:15310/05:00002229 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Production of reactive oxygen species by Photosystem II.

Popis výsledku v původním jazyce

Molecular oxygen released as a by-product of photosynthetic water oxidation acts in two different ways in the photosynthetic processes: 1) O2 is used as a sink for electrons and 2) O2 is a source of reactive oxygen species (ROS). Oxygen is reduced in successive univalent reactions to form superoxide radical (O2 -), hydrogen peroxide (H2O2) and hydroxyl radical (OH ). Production of O2 - was demonstrated using either an assay involving of cytochrome c reduction in the presence of xanthine/xanthine oxidaseor voltametric methods. The production of H2O2 was suggested to originate either from the dismutation of O2 - or incomplete oxidation of water. Using spin-trapping EPR technique it has been demonstrated that illumination of PSII membranes results in theformation of OH . In the present study, EPR spin-trapping spectroscopy was used to investigate light-induced production of ROS in PSII membranes. We have found that O2 - production on PSII acceptor side initiated formation of OH via two

Název v anglickém jazyce

Production of reactive oxygen species by Photosystem II.

Popis výsledku anglicky

Molecular oxygen released as a by-product of photosynthetic water oxidation acts in two different ways in the photosynthetic processes: 1) O2 is used as a sink for electrons and 2) O2 is a source of reactive oxygen species (ROS). Oxygen is reduced in successive univalent reactions to form superoxide radical (O2 -), hydrogen peroxide (H2O2) and hydroxyl radical (OH ). Production of O2 - was demonstrated using either an assay involving of cytochrome c reduction in the presence of xanthine/xanthine oxidaseor voltametric methods. The production of H2O2 was suggested to originate either from the dismutation of O2 - or incomplete oxidation of water. Using spin-trapping EPR technique it has been demonstrated that illumination of PSII membranes results in theformation of OH . In the present study, EPR spin-trapping spectroscopy was used to investigate light-induced production of ROS in PSII membranes. We have found that O2 - production on PSII acceptor side initiated formation of OH via two

Druh

D - Stať ve sborníku

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Photosynthesis: Fundamental Aspects to Global Perspectives (Proceedings of the 13th International Congress on Photosynthesis; CD-ROM).

ISBN

1-891276-39-5

ISSN

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e-ISSN

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Počet stran výsledku

1000

Strana od-do

497-498

Název nakladatele

Allen Press

Místo vydání

Montreal, Kanada

Místo konání akce

Montreal, Kanada

Datum konání akce

1. 1. 2005

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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