Role of chloride ion in hydroxyl radical production in photosystem II under heat stress: Electron paramagnetic resonance spin-trapping study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33142585" target="_blank" >RIV/61989592:15310/12:33142585 - isvavai.cz</a>

Výsledek na webu

<a href="http://download.springer.com/static/pdf/56/art%253A10.1007%252Fs10863-012-9433-4.pdf?auth66=1362665862_679ae7727f40de6fc139c6eddcaa1261&amp;ext=.pdf" target="_blank" >http://download.springer.com/static/pdf/56/art%253A10.1007%252Fs10863-012-9433-4.pdf?auth66=1362665862_679ae7727f40de6fc139c6eddcaa1261&amp;ext=.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10863-012-9433-4" target="_blank" >10.1007/s10863-012-9433-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Role of chloride ion in hydroxyl radical production in photosystem II under heat stress: Electron paramagnetic resonance spin-trapping study

Popis výsledku v původním jazyce

Hydroxyl radical (HO?) generation in photosystem II (PSII) was studied by electron paramagnetic resonance (EPR) spin-trapping technique. It is demonstrated here that the exposure of PSII membranes to heat stress (40 °C) results in HO? formation, as monitored by the formation of EMPO-OH adduct EPR signal. The presence of different exogenous halides significantly suppressed the EMPO-OH adduct EPR signal in PSII membranes under heat stress. The addition of exogenous acetate and blocker of chloride channelsuppressed EMPO-OH adduct EPR signal, whereas the blocker of calcium channel did not affect EMPO-OH adduct EPR signal. Heat-induced hydrogen peroxide (H2O2) production was studied by amplex red fluorescent assay. The presence of exogenous halides, acetate and chloride blocker showed the suppression of H2O2 production in PSII membranes under heat stress. Based on our results, it is proposed that the formation of HO? under heat stress is linked to uncontrolled accessibility of water to the

Název v anglickém jazyce

Role of chloride ion in hydroxyl radical production in photosystem II under heat stress: Electron paramagnetic resonance spin-trapping study

Popis výsledku anglicky

Hydroxyl radical (HO?) generation in photosystem II (PSII) was studied by electron paramagnetic resonance (EPR) spin-trapping technique. It is demonstrated here that the exposure of PSII membranes to heat stress (40 °C) results in HO? formation, as monitored by the formation of EMPO-OH adduct EPR signal. The presence of different exogenous halides significantly suppressed the EMPO-OH adduct EPR signal in PSII membranes under heat stress. The addition of exogenous acetate and blocker of chloride channelsuppressed EMPO-OH adduct EPR signal, whereas the blocker of calcium channel did not affect EMPO-OH adduct EPR signal. Heat-induced hydrogen peroxide (H2O2) production was studied by amplex red fluorescent assay. The presence of exogenous halides, acetate and chloride blocker showed the suppression of H2O2 production in PSII membranes under heat stress. Based on our results, it is proposed that the formation of HO? under heat stress is linked to uncontrolled accessibility of water to the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Bioenergetics and Biomembranes

ISSN

0145-479X

e-ISSN

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Svazek periodika

44

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

"365?372"

Kód UT WoS článku

000304613700009

EID výsledku v databázi Scopus

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