Důkaz podílu cytochromu b559 na produkci superoxidu ve fotosystému II: efekt syntetických krátkých plastochinonů na tabáku s mutovaným cytochromem b559.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00003238" target="_blank" >RIV/61989592:15310/06:00003238 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Evidence that cytochrome b559 is involved in superoxide production in Photosystem II: effect of synthetic short-chain plastoquinones in a cytochrome b559 tobacco mutant.

Popis výsledku v původním jazyce

Light-induced production of superoxide (O2.-) in spinach PSII membrane particles was studied using EPR spin-trapping spectroscopy. The presence of exogenous plastoquinones with a different side chain length enhanced O2.- production in the following order: PQ-1>PQ-2>>PQ-9. In PSII membrane particles isolated from tobacco mutant of cytochrome b559 (cyt b559) carrying single-point mutation in the b-subunit, which has also reduced amount of the a-subunit, the effect of PQ-1 was smaller than in the wild type. The increase in LP cyt b559 content, induced by incubation of spinach PSII membrane particles at low pH, resulted in a significant increase in O2.- formation in the presence of PQ-1, whereas it had little effect on O2.- production in the absence of PQ-1. The enhancement of O2.- formation induced by PQ-1 was not abolished by DCMU. Under anaerobic conditions, the dark oxidation of LP cyt b559 increased as the pH was decreased. The presence of molecular oxygen significantly enhanced dark

Název v anglickém jazyce

Evidence that cytochrome b559 is involved in superoxide production in Photosystem II: effect of synthetic short-chain plastoquinones in a cytochrome b559 tobacco mutant.

Popis výsledku anglicky

Light-induced production of superoxide (O2.-) in spinach PSII membrane particles was studied using EPR spin-trapping spectroscopy. The presence of exogenous plastoquinones with a different side chain length enhanced O2.- production in the following order: PQ-1>PQ-2>>PQ-9. In PSII membrane particles isolated from tobacco mutant of cytochrome b559 (cyt b559) carrying single-point mutation in the b-subunit, which has also reduced amount of the a-subunit, the effect of PQ-1 was smaller than in the wild type. The increase in LP cyt b559 content, induced by incubation of spinach PSII membrane particles at low pH, resulted in a significant increase in O2.- formation in the presence of PQ-1, whereas it had little effect on O2.- production in the absence of PQ-1. The enhancement of O2.- formation induced by PQ-1 was not abolished by DCMU. Under anaerobic conditions, the dark oxidation of LP cyt b559 increased as the pH was decreased. The presence of molecular oxygen significantly enhanced dark

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/1K04103" target="_blank" >1K04103: Reaktivní formy kyslíku ve fotosyntéze</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochemical Journal

ISSN

0264-6021

e-ISSN

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Svazek periodika

397

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

321-327

Kód UT WoS článku

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EID výsledku v databázi Scopus

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