Superoxide oxidase and reductase activity of cytochrome b559 in photosystem II

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010346" target="_blank" >RIV/61989592:15310/09:00010346 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Superoxide oxidase and reductase activity of cytochrome b559 in photosystem II

Popis výsledku v původním jazyce

This study provides evidence for the superoxide oxidase and the superoxide reductase activity of cytochrome b559 (cyt b559) in PSII. It is reported that in Tris-treated PSII membranes upon illumination, both the intermediate potential (IP) and the reduced high potential (HPred) forms of cyt b559 exhibit superoxide scavenging activity and interconversion between IP and HPred form. When Tris-treated PSII membranes were illuminated in the presence of spin trap EMPO, the formation of superoxide anion radical (O2?-) was observed, as confirmed by EPR spin-trapping spectroscopy. The observations that the addition of enzymatic (superoxide dismutase) and non-enzymatic (cytochrome c, ?-tocopherol and Trolox) O2?- scavengers prevented the light-induced conversionof IP?HPred cyt b559 confirmed that IP and HPred cyt b559 is reduced and oxidized by O2?-, respectively. Redox changes in cyt b559 by an exogenous source of O2?- reconfirmed the superoxide oxidase and reductase activity of cyt b559. Furt

Název v anglickém jazyce

Superoxide oxidase and reductase activity of cytochrome b559 in photosystem II

Popis výsledku anglicky

This study provides evidence for the superoxide oxidase and the superoxide reductase activity of cytochrome b559 (cyt b559) in PSII. It is reported that in Tris-treated PSII membranes upon illumination, both the intermediate potential (IP) and the reduced high potential (HPred) forms of cyt b559 exhibit superoxide scavenging activity and interconversion between IP and HPred form. When Tris-treated PSII membranes were illuminated in the presence of spin trap EMPO, the formation of superoxide anion radical (O2?-) was observed, as confirmed by EPR spin-trapping spectroscopy. The observations that the addition of enzymatic (superoxide dismutase) and non-enzymatic (cytochrome c, ?-tocopherol and Trolox) O2?- scavengers prevented the light-induced conversionof IP?HPred cyt b559 confirmed that IP and HPred cyt b559 is reduced and oxidized by O2?-, respectively. Redox changes in cyt b559 by an exogenous source of O2?- reconfirmed the superoxide oxidase and reductase activity of cyt b559. Furt

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica et Biphysica Acta - Bioenergetics

ISSN

0005-2728

e-ISSN

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Svazek periodika

1787

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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