Formation of superoxide anion and carbon-centered radicals by photosystem II under high light and heat stress-EPR spin-trapping study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33145242" target="_blank" >RIV/61989592:15310/13:33145242 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10863-013-9523-y" target="_blank" >http://dx.doi.org/10.1007/s10863-013-9523-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10863-013-9523-y" target="_blank" >10.1007/s10863-013-9523-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Formation of superoxide anion and carbon-centered radicals by photosystem II under high light and heat stress-EPR spin-trapping study

Popis výsledku v původním jazyce

In this study, electron paramagnetic resonance spin-trapping technique was used to study the light-induced production of superoxide anion (O2?-) and carbon-centered (R?) radicals by Photosystem II (PSII). It is evidenced here that exposure of PSII membranes to high light (2000 micromol photons m-2 s-1) or heat (47 °C) treatments prior to the illumination suppressed O2?- production, while R? was formed. Formation of R? in the both high light- and heat-treated PSII membranes was enhanced by DCMU. Removalof molecular oxygen by glucose/glucose oxidase/catalase system and O2?- scavenging by exogenous superoxide dismutase completely suppressed carbon-centered radical formation. It is proposed here that the oxidation of polyunsaturated fatty or amino acid byO2?- on the electron acceptor side of PSII results in the formation of R?, known to initiate a cascade reaction leading to the lipid peroxidation and protein degradation.

Název v anglickém jazyce

Formation of superoxide anion and carbon-centered radicals by photosystem II under high light and heat stress-EPR spin-trapping study

Popis výsledku anglicky

In this study, electron paramagnetic resonance spin-trapping technique was used to study the light-induced production of superoxide anion (O2?-) and carbon-centered (R?) radicals by Photosystem II (PSII). It is evidenced here that exposure of PSII membranes to high light (2000 micromol photons m-2 s-1) or heat (47 °C) treatments prior to the illumination suppressed O2?- production, while R? was formed. Formation of R? in the both high light- and heat-treated PSII membranes was enhanced by DCMU. Removalof molecular oxygen by glucose/glucose oxidase/catalase system and O2?- scavenging by exogenous superoxide dismutase completely suppressed carbon-centered radical formation. It is proposed here that the oxidation of polyunsaturated fatty or amino acid byO2?- on the electron acceptor side of PSII results in the formation of R?, known to initiate a cascade reaction leading to the lipid peroxidation and protein degradation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Bioenergetics and Biomembranes

ISSN

0145-479X

e-ISSN

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Svazek periodika

45

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

551-559

Kód UT WoS článku

000327082100005

EID výsledku v databázi Scopus

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